摘要
采用逆转录聚合酶链式反应(RT-PCR)及蛋白免疫印迹杂交(Western Blot)技术,研究0.5mmol/L亚精胺浸种的黄瓜幼苗在淹水胁迫下,根热激蛋白70基因(HSP70)mRNA和蛋白质的表达量的变化。结果表明:淹水胁迫使黄瓜根HSP70的mRNA和蛋白的表达呈现先上升后下降的趋势,在淹水4h时,HSP70的mRNA和蛋白表达量均极显著高于未淹水处理;亚精胺浸种的黄瓜根HSP70的mRNA和蛋白的表达量在24h内呈一直上升的趋势,在淹水24h时,HSP70的mRNA和蛋白表达量均极显著高于未淹水处理。淹涝胁迫下,亚精胺浸种的黄瓜根HSP70的mRNA和蛋白表达量在淹水12h和24h时极显著高于蒸馏水浸种。外源亚精胺能诱导淹涝胁迫下黄瓜幼苗根HSP70mRNA和蛋白质的表达量的增加,缓解淹涝胁迫对黄瓜造成的伤害。
RT-PCR and Western Blot were adopted to study the effects of exogenous spermidine(0.5mmol/L)on HSP70in mRNA and protein expression of cucumber roots under waterlogging stress.The results showed that under waterlogging stress,the expression of HSP70in cucumber roots soaked with distilled water at mRNA and protein level increased and then decreased with the extension of waterlogging time,when the waterlogging time was up to 4h,the expression quantity of HSP70mRNA and protein were extremely higher compared with no waterlogging treatment;the expression of HSP70in cucumber roots soaked with exogenous spermidine at mRNA and protein level increased within 24h,when the waterlogging time was up to 24h,the expression quantity of HSP70mRNA and protein were extremely significant different compared with no waterlogging treatment.Under waterlogging stress,the expression of HSP70mRNA and protein cucumber roots soaked with exogenous spermidine were extremely higher when the waterlogging time was up to 12hand 24hcompared with soaked with distilled water.Exogenous spermidine could induce the expression of HSP70and protein in cucumber roots increased under waterlogging stress,and could relieve the harm caused by waterlogging stress.
出处
《广西植物》
CAS
CSCD
北大核心
2013年第5期669-673,共5页
Guihaia
基金
转基因生物新品种重大专项
转基因生物分子特征识别技术(2011ZX08012-002)
江苏高校优势学科建设工程资助项目
徐州师范大学科研基金(08XLA08)
