摘要
采用营养液水培方式,研究了根际低氧胁迫下外源多胺对黄瓜幼苗植株根系生长,内源多胺含量与质膜H+-ATP酶、液泡膜H+-ATP酶和焦磷酸酶活性的影响。结果表明,根际低氧胁迫显著抑制黄瓜幼苗根系的生长,外源Put(腐胺)和Spd(亚精胺)可缓解低氧胁迫对根系的生长抑制,多胺主要以Spd的形式发挥促进性的生理作用,Put通过转化为Spd发挥作用;低氧胁迫下黄瓜根系内源多胺含量略有提高,外源多胺处理可增加内源多胺的含量;低氧胁迫下外源Put和Spd处理后质膜H+-ATP酶活性显著提高,外源多胺对黄瓜根系液胞膜H+-ATP酶和H+-焦磷酸酶活性没有明显影响,说明低氧胁迫下外源多胺主要通过提高质膜H+-ATP酶活性而发挥生理作用。
The effects of exogenous polyamines on the growth, polyamines contents, the activities of H^＋ -ATPase and H^＋ -PPase of plasma membrane and tonoplast in cucumber seedling roots under root-zone hypoxia stress were Studied under hydroponic culture. The results showed that the growth of cucumber roots was significantly inhibited by hypoxia stress （Table 1）. While the growth inhibition in cucumber roots by hypoxia stress could be alleviated by spraying exogenous polyamines. From these results （Tables 1 and 2） we can conclude that Spd played an active physiological role in relieving hypoxia stress and Put exerted actively physiological role by enhancing Spd biosynthesis. The results also showed that the endogenous polyamine contents in cucumber roots were increased by hypoxia stress and spraying exogenous polyamines. Exogenous polyamines application obviously promoted the H^＋ -ATPase activities of plasma membrane in cucumber roots （Fig. 1）, but did not significantly affect the H^＋ -ATPase and H^＋ -PPase activities of tonoplast in cucumber roots （Figs.2,3）. The resuits suggested that exogenous polyamines could enhance hypoxia resistance of cucumber seedlings by increasing H^＋ -ATPase activities of plasma membrane in cucumber roots.
出处
《植物生理与分子生物学学报》
CAS
CSCD
北大核心
2005年第6期637-642,共6页
Journal Of Plant Physiology and Molecular Biology
基金
国家自然科学基金项目(Nos.30170645
30571263)
江苏省自然科学基金项目(No.BK2002110)资助。~~
