摘要
目的:探讨不同海拔地区正常人群血清氧化应激反应相关指标含量变化。方法:采用比色法检测不同海拔地区(高海拔组、中度海拔组、平原组)血清丙二醛(malondialdehyde,MDA)、羟自由基(hydroxyl radicals,OH)、总抗氧化能力(total antioxidant capacity,T-AOC)、超氧化物歧化酶(superoxyde dismutase,SOD)、还原型谷胱甘肽(reduced glutathion,GSH)、氧化型谷胱甘肽(oxidized glutathione,GSSG)的含量。结果:高海拔组血清MDA、OH含量明显高于中度海拔组、平原组(9.91±9.17)nmol/L vs(5.71±1.00)nmol/L、(3.72±0.60)nmol/L(均P<0.05);(576.46±307.04)U/mL vs(361.79±312.61)U/mL、(198.31±109.37)U/mL(均P<0.05);中度海拔组血清MDA、OH含量明显高于平原组(5.71±1.00)nmol/L vs(3.72±0.60)nmol/L(P<0.05);(361.79±312.61)U/mL vs(198.31±109.37)U/mL(P<0.05);平原组血清AOC、SOD、GSH/GSSG含量明显高于中度海拔组及高海拔组(21.84±5.85)U/mL vs(15.05±5.16)U/mL、(13.33±4.05)U/mL(均P<0.05);(56.8±13.75)U/mL vs(40.38±23.98)U/mL、(21.75±5.86)U/mL(均P<0.05);(19.07±13.71)μmo/L vs(7.41±2.48)μmo/L、(6.39±1.92)μmo/L(均P<0.05)。中度海拔组血清AOS、SOD、GSH/GSSG含量明显高于高海拔组(15.05±5.16)U/mL vs(13.33±4.05)U/mL(P<0.05);(40.38±23.98)U/mL vs(21.75±5.86)U/mL(P<0.05);(7.41±2.48)μmo/L vs(6.39±1.92)μmo/L(P<0.05)。结论:随着海拔的升高,正常人群氧化应激反应升高,抗氧化能力下降。
Objective：This paper is to explore the content changes of normal people＇s serum oxidative stress related indicators in different altitude regions.Methods：Contents of serum MDA,OH,T-AOC,SOD,GSH and GSSG in different altitude regions（High altitude group,Moderate altitude group,Plain group）are detected by colorimetric method.Results：Contents of serum MDA and OH in High altitude group are higher than moderate altitude group and plain group（9.91±9.17）nmol/L vs（5.71±1.00）nmol/L and（3.72±0.60）nmol/L（P〈0.05）,（576.46±307.04）U/mL vs（361.79±312.61）U/mL（198.31±109.37）U/mL（P〈0.05）.Contents of serum MDA and OH in moderate altitude group is higher than plain group（5.71±1.00）nmol/L vs（3.72±0.60）nmol/L（P〈0.05）,（361.79±312.61）U/mL vs（198.31±109.37）U/mL（P〈0.05）.Contents of serum TAOC,SOD and GSH/GSSG in plain group are higher than moderate altitude group and high altitude group（21.84±5.85）U/mL vs（15.05±5.16）U/mL and（13.33±4.05）U/mL（P〈0.05）,（56.8±13.75）U/mL vs（40.38±23.98）U/mL and（21.75±5.86）U/mL（P〈0.05）;（19.07±13.71）μmo/L vs（7.41±2.48）μmo/L and（6.39±1.92）μmo/L（P〈0.05）.Contents of serum T-AOC,SOD and GSH/GSSG in moderate altitude group are higher than High altitude group（15.05±5.16）U/mL vs（13.33±4.05）U/mL（P〈0.05）;（40.38±23.98）U/mL vs（21.75±5.86）U/mL（P〈0.05）;（7.41±2.48）μmo/L vs（6.39±1.92）μmo/L（P〈0.05）.Conclusion：With the elevation increasing,oxidative stress reaction of normal people increases,and their antioxidant capacity decreases.
出处
《高原医学杂志》
CAS
2017年第3期13-15,共3页
Journal of High Altitude Medicine
基金
青海省科技厅科研项目(NO:2009-Z-726)
关键词
不同海拔地区
正常人群
氧化应激
Different altitude regions
Normal people
Oxidative stress
