摘要
目的:建立苍耳子药材的HPLC特征图谱,为评价不同产地苍耳子的质量提供参考。方法:采用Alltima C18(250 mm×4.6 mm,5μm),流动相乙腈-0.1%磷酸溶液梯度洗脱,流速:0.8 ml·min^(-1),检测波长:278 nm,柱温:25℃,对来自河南、吉林、安徽等地共47批苍耳子样品运用"中药指纹图谱相似度评价系统"进行分析。结果:以绿原酸为参照峰,初步构建了由5个特征峰组成的苍耳子HPLC特征图谱。结论:该方法简便、可靠,为苍耳子药材的质量控制提供了有效的评价方法。
Objective： To establish the HPLC specific chromatogram and provide comprehensive evaluation of Xanthiifructus from different regions. Methods： The HPLC analysis was performed on an Alltima C18 column （250 mm×4.6mm,5μm） with acetonitrile- 0.1% phosphoric acid solution as the mobile phase with gradient elution at a flow rate of 0.8 ml min-l. The detection wavelength was 278 nm. The column temperature was 25 %. The software ＂Similarity Evaluation System for Chromatographic Fingerprint of TCMs＂ was employed to carry out the similarity analysis of the samples from Henan, Jilin, Anhui and the other regions. Results： The specific chromatogram was preliminarily constructed and 5 common peaks with chlorogenic acid as the reference were identified. Conclusion： The method is scientific basis of the quality assessment of Xanthii fructus with convenient and reliable properties.
出处
《中国药师》
CAS
2018年第1期71-74,共4页
China Pharmacist
基金
中央本级重大增减支项目(编号:2060302)
