摘要
目的建立乌梅生品与炭品中有效成分的特征图谱,为乌梅饮片的质量评价提供新的依据。方法采用VenusilXBPC18色谱柱(4.6mm×250mm,5μm);流动相:0.5%磷酸二氢铵溶液-乙腈(97:3)等度洗脱;流速1.0mL/min;柱温25℃;检测波长210nm。结果 9批乌梅生品显示6个共有特征峰,9批乌梅炭品显示8个共有特征峰,标定了其中3个特征成分(柠檬酸、苹果酸、没食子酸)。结论建立的乌梅生品及其炭品的HPLC特征图谱,可有效地评价乌梅生品及其炭品的质量。
Objective To establish a specific chromatogram of the active ingredients of dark plum and its carbon products, and to provide a new basis for the quality evaluation of the dark plum pieces. Methods Chromatographic column： Venusil XBP C18 （4.6mm × 250mm, 5μm）;mobile phase： 0.5% phosphoric acid-two ammonium hydrogen solution - acetonitrile （97： 3） isocratic elution;flow rate： 1.0mL/min;column temperature： 25℃ ;detection wavelength： 210nm.Results The 9 batches of dark plum showed 6 common characteristic peaks, the 9 batches of its carbon products showed 8 common characteristic peaks, the calibration of which three characteristic components （ citric acid, malic acid, gallic acid） were demarcated. Conclusion The HPLC specific chromatogram can be used to evaluate the quality of dark plum and its carbon products, which provides a new basis for the development of the ideal quality system of dark plum and its carbon products.
作者
叶静
付静
余晗
刘艺莹
聂诗明
YE Jing;FU Jing;YU Han;LIU Yiying;NIE Shiming(School of Pharmacy, Hubei University of Chinese Medicine, Wuhan 43006)
出处
《湖北中医药大学学报》
2017年第6期42-45,共4页
Journal of Hubei University of Chinese Medicine
基金
国家科技部科技基础性专项(项目编号:2014FY111100-2)
关键词
乌梅
乌梅炭
HPLC特征图谱
枸橼酸
苹果酸
没食子酸
dark plum
dark plum carbon products
HPLC specific chromatogram
citrate
malic acid
gallic acid
