摘要
为明确不同冬春性小麦品种在相同穗分化进程下对低温的生理响应,选用冬性品种烟农19和春性品种宁麦4号、扬糯麦1号、宁麦13、扬麦11、镇10375,研究了-10℃人工低温胁迫下,护颖分化期、雌雄蕊分化期、药隔分化期和四分体形成期叶片中可溶性蛋白含量、丙二醛含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶活性变化。结果表明,低温处理后,4个穗分化时期的可溶性蛋白含量和抗氧化酶活性均较0 h对照升高,但随着低温处理时间延长而下降;丙二醛含量呈先升后降再上升的趋势。药隔分化期对低温胁迫最为敏感。四分体形成期,叶片生理响应不宜作为评价抗寒性的指标。与0 h对照相比,烟农19的可溶性蛋白含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶活性在低温处理后,上升幅度和响应速率大于其他5个春性品种,且丙二醛含量的增幅较小,对低温胁迫表现出较强抗性。
In order to define the physiological response of leal to freezing temperature of different wheat cultivars during the same period of young spike development,the winterness cultivar Yannong 19 and springness cultivars Ningmai 4 ,Yangnuomai 1 ,Ningmai 13,Yangmai 11,Zhenmai1O375 were studied for the content of soluble protein and malondialdehyde （ MDA）,as well as the activities of su-peroxide dismutase （ SOD） ,peroxidase （ POD） and cata-lase （ CAT）,with the leaves treated under the low temper-ature stress of - 10℃ during the glume differentiation stage, pistil and stamen differentiation stage, anther differ-entiation stage and the tetrad formation stage. The results showed that the soluble protein content and antioxidant ac-tivity during the four stages in the treatment of low temper-ature increased compared with the control, but decreased with the prolongation of the treatment. The content of MDA increased firstly, then decreased, and finally increased. Leaves of wheat in anther differentiation stage were most sensitive to low temperature stress. While the physiological response of leaves, which in the tetrad formation stage, should not be used to evaluate the cold resistance of wheat. Compared with the control, the soluble protein content, SOD, POD and CAT activities of Yannong 19 were higher than those of the other five cultivars after low temperature treatment, and the increase of MDA content was small. Thus the resistance to low tempera-ture stress showed by Yannong 19 was the strongest in these six cultivars.
出处
《江苏农业学报》
CSCD
北大核心
2017年第6期1212-1219,共8页
Jiangsu Journal of Agricultural Sciences
基金
国家现代农业产业技术体系建设专项(CARS-3-1-1
CARS-3-2-11)
扬州市绿扬金凤人才计划项目(yzlyjfjh2015YB109)
江苏里下河地区农业科学研究所科研基金项目(lxh201504)
扬州市现代农业项目(YZ2016033)
关键词
小麦
低温
幼穗分化
生理特性
wheat
low temperature
spike differentiation
physiological characteristics
