摘要
为探讨春季低温(倒春寒)胁迫下抗倒春寒能力不同的小麦品种相关miRNA及其响应规律,提供miRNA的表达谱和差异表达miRNA的信息,揭示差异表达miRNA在小麦抗倒春寒中的作用,采用高通量测序(Small RNA-seq)技术对雌雄蕊原基分化期0℃低温胁迫72 h及未低温处理(对照)的矮抗58(AK58)和郑麦366(ZM366)幼穗进行测序,通过生物信息学分析,以padj<0.05且|log2(Fold_change)|≥1为条件,筛选低温胁迫下差异表达显著的miRNA,利用psRobot(v1.2)对差异表达miRNA靶基因进行预测,然后对差异表达显著miRNA的靶基因进行GO富集分析和KEGG Pathway分析。结果表明,小麦幼穗测序共鉴定到112类miRNA,分属于38个不同的MIRNA家族。AK58、ZM366分别鉴定出85,88个差异表达miRNA,AK58有27个miRNA表达有显著差异,其中23个显著上调表达,4个显著下调表达,差异表达极显著的miRNA为2个,分别是tae-miR9672b、tae-miR9672a-3p;ZM366有48个差异表达显著的miRNA,13个显著上调表达,35个显著下调表达,差异表达极显著的miRNA为4个,分别为tae-miR9672b、tae-miR9672a-3p、tae-miR6201、tae-miR9674b-5p。对低温胁迫下AK58与ZM366差异表达的miRNA进行靶基因预测,AK5885个差异表达miRNA对应848个靶基因;ZM36688个差异表达miRNA对应6537个靶基因。GO功能富集分析结果显示,AK58差异表达miRNA靶基因有20个生物学过程、6个细胞成分、6个分子功能类别极显著富集(FDR<0.01);ZM366有10个生物学过程、14个细胞成分、19个分子功能类别极显著富集(FDR<0.01)。对2个小麦品种幼穗中差异表达miRNA靶基因进行KEGG代谢通路(Pathway)富集分析,AK58差异表达miRNA靶基因显著富集(P<0.05)到RNA聚合酶,嘌呤代谢,嘧啶代谢,光合生物固碳途径,自噬,托烷、哌啶和吡啶生物碱的生物合成,核苷酸切除修复,错配修复,DNA复制,核糖体,烟酸和烟酰胺代谢11个代谢通路;ZM366差异表达miRNA靶基因显著富集(P<0.05)在植物-病原互作,
To explore the relevant miRNAs and their response laws of wheat varieties with different resistance to cold in spring under the stress of low temperature in spring(late spring cold),high-throughput sequencing(Small RNA-seq)technology was used to analyze the young panicles of Aikang 58(AK58)and Zhengmai 366(ZM366)in the differentiation stage of pistil and stamen primordium at 0℃low temperature stress for 72 h and without low temperature treatment(control).The purpose of sequencing is provide miRNA expression profiles and information on differentially expressed miRNAs,and reveal the role of differentially expressed miRNAs in wheat resistance to late spring cold.Through bioinformatics analysis,under the condition of padj<0.05 and|log2(Fold_change)|≥1,the miRNAs with significant differential expression was screened under low temperature stress;using psRobot(v1.2)to predict the differentially expressed miRNA target genes,and then target genes with significantly differentially expressed miRNAs were subjected to GO enrichment analysis and KEGG Pathway analysis.The results showed that 112 types of miRNAs were identified by sequencing of wheat ears,belonging to 38 different MIRNA families.AK58 and ZM366 identified 85 and 88 differentially expressed miRNAs,respectively.AK58 had 27 miRNAs with significant differences in expression,23 of which were significantly up-regulated,4 were significantly down-regulated,and 2 miRNAs with extremely significant differential expression were tae-miR9672b,tae-miR9672a-3p;ZM366 had 48 miRNAs with significant differential expression,13 with significantly up-regulated expression,35 with significantly down-regulated expression,4 miRNAs with extremely significant differential expression,tae-miR9672b,tae-miR9672a-3p,tae-miR6201,tae-miR9674b-5p.Target genes were predicted for the differentially expressed miRNAs of AK58 and ZM366 under low temperature stress.85 differentially expressed miRNAs of AK58 correspond to 848 target genes;88 differentially expressed miRNAs of ZM366 correspond to 6537
作者
张自阳
王斌
王智煜
王志伟
朱启迪
茹振钢
刘明久
ZHANG Ziyang;WANG Bin;WANG Zhiyu;WANG Zhiwei;ZHU Qidi;RU Zhengang;LIU Mingjiu(Henan Institute of Science and Technology,Collaborative Innovation Center of Modern Biological Breeding,Xinxiang 453003,China)
出处
《华北农学报》
CSCD
北大核心
2021年第1期81-94,共14页
Acta Agriculturae Boreali-Sinica
基金
科技部创新方法专项(2018IM030100)
河南省基础与前沿技术研究计划项目(162300410142)
河南省科技攻关项目(212102110298)。
