摘要
目的研究冬凌草甲素对糖尿病大鼠肾脏的保护作用。方法将24只SD大鼠随机分正常对照组(NC)、糖尿病组(DM)、冬凌草甲素组(DO),DM组和DO组大鼠用腹腔注射链脲佐菌素制备糖尿病模型。DO组大鼠给以冬凌草甲素10 mg/kg灌胃,NC组和DM组大鼠均给予等量的生理盐水灌胃。3组大鼠干预给药8周后,检测24 h尿蛋白、血肌酐(SCr)、尿素氮(BUN)、三酰甘油(triglyceride,TG)、总胆固醇(total cholesterol,TC)、高密度脂蛋白(high density lipoprotein cholesterol,HDL)、低密度脂蛋白(low density lipoprotein cholesterol,LDL);PAS染色观察肾组织病理学改变;试剂盒检测肾组织匀浆中超氧化物歧化酶(superoxide dismutase,SOD)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)活性及丙二醛(malonic dialdehyde,MDA)含量;用免疫印迹试验和逆转录聚合酶链反应检测SOD蛋白和mRNA表达情况。结果糖尿病大鼠24 h尿蛋白、SCr、BUN、TC、TG、LDL水平升高(P<0.05),HDL降低,肾组织病理肾小球肥大,肾组织SOD和GSH-Px活力下降(P<0.05),SOD蛋白和mRNA表达下降,MDA含量升高(P<0.05)。冬凌草甲素处理后可部分逆转肾功能,减轻肾组织损伤;提高SOD、GSH-Px活力,并增加SOD蛋白和mRNA表达,降低MDA含量,即调节氧化应激;冬凌草甲素处理降低TC、TG、LDL水平,HDL升高,即改善脂代谢(P<0.05)。结论冬凌草甲素能通过抑制氧化应激和改善脂代谢减轻糖尿病大鼠肾损伤。
Objective To investigate the protective effect and mechanism of oridonin on kidney of diabetic rats. Methods SD rats were randomly divided into three groups（ n = 8 per group） as follows： normal group（ NC）,diabetic mellitus group（ DM）,diabetic mellitus treated with oridonin group（ DO）. The rat diabetic model was established by intraperitoneal injection of STZ once. Oridonin was administered by gavage to the rats of DO group at a dose of 10 mg/kg/day for 8 weeks. The rats in NC group and DM group were treated with equal volume of normal saline. The animals were sacrificed after 8 weeks of treatment with oridonin. The 24-h urinary protein,serum creatinine（ SCr）,blood urea nitrogen（ BUN）,triglyceride（TG）,total cholesterol（TC）,high-density lipoprotein（HDL）,low-density lipoprotein（LDL）,and the activity of superoxide dismutase（ SOD）,glutathione peroxidase（ GSH-Px） and the content of malondialdehyde（ MDA） in renal tissues were determined. Western blotting and RT-PCR were used to detect the protein and mRNA expression of SOD. PAS staining was used to observe the pathological changes of renal tissues.Results As compared with NC group,DM group had significantly increased proteinuria,SCr,BUN,TG,TC and LDL,and decreased HDL. The content of MDA was increased but the activity of SOD and GSH-Px were decreased in renal tissues in DM group（ P〈0. 05）. The protein and mRNA expression of SOD was decreased in renal tissues in DM group（ P〈0. 05）. Oridonin treatment could reverse the renal function and renal histopathological changes,decrease the content of MDA,and increase the activity of SOD and GSH-Px（P〈0. 05）. The protein and mRNA expression of SOD was also up-regulated in renal tissues of rats treated with oridonin. And the levels of TG,TC and LDL were decreased,and the level of HDL was increased in plasma under the treatment of oridonin（ P〈0. 05）. Conclusions Oridonin relieves renal damage of diabetic rats through modulation of oxidative stress and lipid
出处
《临床肾脏病杂志》
2017年第11期692-696,共5页
Journal Of Clinical Nephrology
基金
国家自然科学基金(No.81170679)
关键词
冬凌草甲素
氧化
脂代谢
糖尿病肾病
Oridonin
Oxidative stress
Lipid metabolism
Diabetic nephropathy
