摘要
目的:考察多发性骨髓瘤细胞系来源外泌体对NK细胞表面活化受体的影响,从外泌体水平探讨骨髓瘤患者中NK细胞功能缺陷的机制。方法:采用超速离心法提取多发性骨髓瘤细胞系RPMI 8226、U266培养上清液中外泌体,并采用电子显微镜鉴定外泌体的大小;提取人原代NK细胞,将40μg/ml外泌体与NK细胞共培养后,于0、1、4和24 h用流式细胞术检测NK细胞表面活化受体NKp30、NKG2D和NKp46的表达。结果:在电子显微镜下外泌体呈现囊泡状,大小30-100 nm;骨髓瘤细胞来源外泌体与NK细胞共培养后,NK细胞表面活化受体表达均有不同程度下降。结论:多发性骨髓瘤来源外泌体可以抑制NK细胞表面活化受体的表达。
Objective： To investigate the effect of myeloma-derived exosomes on surface activating receptors of NK cells,and to explore the mechanism of the function defect of NK cells. Methods： The exosomes from the supernatant of multiple myeloma cell lines RPMI8226 and U266 were extracted by ultracentrifugation,and the size of them was identified under electron microscope; the human primary NK cells were extracted,and were co-cultured with the myeloma-derived exosomes（ 40 μg/ml）,then the expression levels of surface activating receptors NKp46,NKp30 and NKG2D of NK cells at 0,1,4 and 24 hours were detected by flow cytometry. Results： The exosomes showed small vesicular,sized 30-100 nm under electron microscope. The expression of surface activating receptors of NK cells declined at different degree after cocultured with myeloma-derived exosomes. Conclusion： Myeloma-derived exosomes can inhibit the expression of surface activating receptors of NK cells.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第6期1713-1717,共5页
Journal of Experimental Hematology
基金
2015年深圳市科技计划项目(JCYJ20150330102401094)
