摘要
目的探讨人白细胞介素6(interleukin-6,IL-6)在结直肠癌患者中的水平及意义。方法收集132例首诊结直肠癌患者(CRC)、35例炎症性肠病(IBD)、31例肠道良性疾病患者(CBD)及84例健康体检者(HC)血清,采用电化学发光双抗体夹心免疫分析法(ECLIA)检测血清中白细胞介素6(IL-6)及癌胚抗原(CEA)水平,分析IL-6水平与结直肠癌患者临床病理特征的相关性;应用受试者工作特性曲线(ROC)和二元Logistic法回归分析IL-6和CEA两指标对结直肠癌的诊断价值;对随访资料完整的120例中晚期结直肠癌患者动态观察治疗前后血清IL-6和CEA水平,分析两指标与肿瘤疗效的关系。结果结直肠癌患者血清IL-6水平显著高于炎症性肠病组(P<0.05)、肠道良性疾病组(P<0.01)和健康对照组(P<0.01),结直肠癌患者血清CEA水平显著高于炎症性肠病组(P<0.05)、肠道良性疾病组(P<0.05)和健康对照组(P<0.01),差异均有统计学意义。CRC患者血清IL-6水平与肿瘤直径、分化程度、组织类型、淋巴结转移、远处转移、TNM分期均显著相关(P<0.05),而与年龄、性别及肿瘤发生部位无明显相关。IL-6诊断结直肠癌的灵敏度(72.7%)和准确性(78.6%)均高于CEA(分别为68.2%和77.9%),特异性(85.2%)低于CEA(88.9%),两指标联合检测能够提高灵敏度(97.2%)和准确性(85.6%)。结直肠癌肿瘤控制组(CR+PR+SD)治疗后两指标均较治疗前有显著下降(P<0.05),差异有统计学意义,而肿瘤进展组(PD)治疗后两指标均未显著下降(P>0.05)。结论 IL-6和CEA两指标联合检测有助于结直肠癌的诊断和疗效观察。
Objective To investigate the expression of serum interleukin-6 （IL-6） in colorectal cancer patients and the relationship between the IL-6 level and the tumor curative effect. Methods The electrochemiluminescence immunoassay was used to examine the serum level of IL-6 and carcino-embryonic antigen （CEA） in 132 colorectal cancer patients （ CRC）, 35 inflammatory bowel disease （ IBD）, 31 colorectal benign disease（CBD） and 84 healthy donors as healthy control group（HC）. The relationship between IL-6 and clinicopathological features of colorectal cancer was analyzed, and ROC curve and binary losistic regression were applied to compare the diagnostic value of the IL-6 and CEA. The dynamic levels of serum IL-6 and CEA before and after treatment in 120 advanced CRC patients with complete case data was observed,and the relationship between IL-6 and CEA and the cruative effect of the tumor was analyzed. Results Both the serum IL-6 and CEA levels of the CRC were higher than IBD （ P 〈 0. 05, P 〈 0. 05, respectively）, CBD （ P 〈 0.01, P 〈 0.05, respectively ）, and HC groups （ P 〈 0.01, P 〈 0.01, respectively）. Serum levels of the IL-6 was significantly correlated with tumor size, degree of tumor differentiation, pathological type, lymph node metastasis, distant metastasis and TNM stage （ all the above, P 〈 0.05 ）. The sensitivity and accuracy of IL-6 for CRC diagnosis were higher than CEA（72, 7% vs 68.2% ,78.6% vs 77.9% ,respectively）,while specifity was lower than CEA（85.2% vs 88.9% ）. The combined detection of IL-6 and CEA were highest in sensitivity （97.2%） and accuracy （85.6%）. After treatment, both the serum IL- 6 and CEA levels significantly decreased in CR ＋ PR ＋ SD group （ both P 〈 0.05 ）, but not in the PD group （both P 〉 0.05 ）. Conclusion The combined detection of serum IL-6 and CEA could be a useful potential diagnostic and theraoeutic evaluation method for colorectal cancer.
出处
《标记免疫分析与临床》
CAS
2017年第11期1274-1279,共6页
Labeled Immunoassays and Clinical Medicine
