摘要
目的:研究不明原因不育男性精子顶体酶活性与精子膜表面抗体水平的关系。方法:采用改良Kennedy法检测214例不明原因不育男性的精子顶体酶活性,并利用混合抗球蛋白反应试验(MAR)检测其精子膜表面抗体水平,同时分析精子顶体酶活性与精子膜表面抗体的相关性。结果:214例不明原因不育男性中精子顶体酶活性正常者158例(对照组)、异常者56例(异常组);异常组精子膜表面抗体水平明显高于对照组(P<0.05);不明原因不育男性精子顶体酶活性与精子膜表面抗体水平呈负相关(r=-0.184,P<0.05)。结论:精子顶体酶活性降低可能是不明原因不育男性生育能力降低的原因之一,且精子膜表面抗体的存在可能对精子顶体酶活性有一定的影响。
Objective： To investigate the relationship between sperm acrosin activity and level of spermatozoa antibody coating in unexplained male infertility. Methods：The sperm acrosin activity of 214 males with unexplained infertility was deteeted by modified Kennedy method, and the level of sperm membrane antibody by mixed antiglobulin reaction test （MAR）.The correlation between sperm aerosin activity and antibody coating of spermatozoa was analyzed. Results：The sperm acrosin activity in 214 unexplained infertile males tested normal in 158 cases （ control group） and abnormal in 56 cases （ abnormal group）. The level of antibody coating of spermatozoa was significantly higher in the abnormal group than that in the control group （P〈0.05）.There was a negative correlation between sperm acrosin activity and level of spermatozoa antibody coating （r =-0. 184, P〈 0.05）. Conclusion： Redueed sperm aerosin activity may be one of the causes for low fertility in males. The presence of antibody coating of spermatozoa may have a certain effect on sperm acrosin activity.
出处
《广州医科大学学报》
2017年第1期10-12,共3页
Academic Journal of Guangzhou Medical University
基金
广州市科技计划项目(2014J4100024)
广州医科大学青年基金资助项目(2013A20)
关键词
不明原因不育
顶体酶
精子膜表面抗体
unexplained infertility
acrosin
antibody coating of spermatozoa
