摘要
采用荧光光谱法、紫外可见吸收光谱法以及同步荧光光谱法研究了米格列奈钙与牛血清白蛋白(BSA)之间的相互作用。实验结果表明,米格列奈钙对牛血清白蛋白的荧光有较为明显的猝灭作用,其猝灭机制为动态猝灭,遵循Stern-Volmer方程;根据F9rster的非辐射能量转移理论,计算出米格列奈钙分子与牛血清白蛋白分子的结合距离为5.461nm;用同步荧光光谱技术探究了米格列奈钙对BSA构象的影响,结果发现,当激发和发射波长差为60nm时,荧光峰位发生了微小的蓝移,说明BSA色氨酸残基附近的外围微环境受到了米格列奈钙分子的影响,极性减弱,BSA的疏水性增强。
The interaction between mitiglinide calcium and bovine serum albumin （BSA） is studied by fluorescence emission spectroscopy, UV-visible absorption spectroscopy and synchronous fluorescence spectroscopy. Experimental results show that mitiglinide calcium leads to the fluorescence quenching of BSA, and the quenching mechanism is a dynamic quenching procedure according to the Stern-Volmer equation. The binding distance between mitiglinide calcium and BSA is calculated to be about 5. 461 nm based on Forster theory. The effect of mitiglinide calcium on the conformation of BSA is studied by synchronous fluorescence spectroscopy. The results show that a slight blue shift is observed when the difference between excitation wavelength and emission wavelength is 60 nm. The blue shift indicates that the peripheral microenvironment near tryptophan residue of BSA is affected by the mitiglinine calcium molecule, which makes the polarity of the microenvironment decrease and the hydrophobieity of BSA increase.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2017年第11期367-373,共7页
Acta Optica Sinica
基金
国家自然科学基金面上项目(61378037)
江苏省自然科学基金(BK20150807)
中央高校基本科研业务费专项资金项目(2014B04714)
中央高校科技发展前瞻性研究专项(2017B42414)
关键词
光谱学
光学
荧光猝灭
荧光光谱
米格列奈钙
牛血清白蛋白
蛋白质构象
spectroscopy
optics
fluorescence quenching
fluorescence spectrum
mitiglinide calcium
bovine serum albumin
protein conformation
