摘要
旨在研究白藜芦醇(Resveratrol,RES)对高脂诱导大鼠的生长、血清生化指标、抗氧化以及肠道形态结构的影响。选取6周龄的雄性SD大鼠25只,将大鼠随机分为5组,每组5个重复,每个重复1只。分别饲喂基础饲粮、基础饲粮+低剂量RES(30mg·(kg BW)^(-1))、高脂饲粮、高脂饲粮+低剂量RES(30mg·(kg BW)^(-1))和高脂饲粮+高剂量RES(60mg·(kg BW)^(-1)),试验期4周。结果表明:1)与基础饲粮组相比,高脂饲粮组大鼠体重显著增加(P<0.05),而基础饲粮+低剂量RES组大鼠体重未发生显著变化(P>0.05);与高脂饲粮组相比,高脂饲粮添加低或高剂量白藜芦醇均降低了大鼠体重,但未达到显著水平(P>0.05),而在高脂饲粮中添加低剂量白藜芦醇显著降低了大鼠采食量(P<0.05)。2)与基础饲粮组相比,高脂饲粮组大鼠血清中尿素氮水平和脂肪酸合成酶(FAS)活性显著降低(P<0.05);与高脂饲粮组相比,高脂饲粮添加高剂量白藜芦醇均显著降低大鼠血清中甘油三酯(TG)、非酯化脂肪酸(NEFA)含量(P<0.05),显著升高了高密度脂蛋白(HDL)的含量(P<0.05)。3)在基础饲粮中添加低剂量白藜芦醇显著降低了大鼠血清中GSH-Px的活力(P<0.05);与高脂饲粮组相比,高脂饲粮添加低或高剂量白藜芦醇均能显著降低大鼠血清MDA浓度(P<0.05),提高T-SOD活性及T-AOC(P<0.05)。4)与高脂饲粮组相比,高脂饲粮添加低或高剂量白藜芦醇显著提高了大鼠空肠隐窝深度(P<0.05),且高脂饲粮添加高剂量白藜芦醇有提高绒毛高度的趋势(P<0.1)。综上表明,白藜芦醇可以有效缓解高脂饲粮导致的氧化损伤,促进大鼠生长及其对饲粮养分的利用,改善血脂代谢,提高机体的抗氧化力,但对肠道形态结构未表现出明显的修复效果。
This study was conducted to investigate the effects of resveratrol （RES） on the growth, serum biochemical indexes, antioxidant capacity and intestinal morphology in rats fed with high fat diet. Twenty-five male Sprague-Dawley rats of 6 weeks of age were randomly divided into 5 groups with 5 replicates of 1 rat each. The rats in the 5 groups were respectively fed a basal diet, basal diet ＋ low dose RES （30 mg·（kg BW）^-1）, high fat diet, high fat diet＋low dose RES（30 mg·（kg BW）^-1）, high fat diet;high dose RES（60 mg·kg BW）^-1）. The trial lasted for 4 weeks. The results showed as follows. 1）Compared with the basal diet group, the body weight of the rats in the high fat diet group significantly increased （P〈0.05）, but there was no signifi- cant difference between the basal diet and basal diet ＋low dose RES groups （P〉0. 05）. Compared to the high fat diet group, low or high dose resveratrol supplemented in high fat diet decreased the body weight of the rats, but the changes were not significant（P〉0.05）, while the addition of low-dose resveratrol in high fat diet significantly reduced the feed intake （P〈0.05）. 2）Compared with the basal diet group, the levels of urea nitrogen and the activity of fatty acid synthase（FAS） in the serum of rats in the high fat diet group were significantly decreased （P〈0.05） ;Compared with the high fat diet group, the addition of high dose of resveratrol in the high fat diet significantly reduced the levels of triglyceride （TG） and non esterified fatty acid （NEFA） （P%0. 05）, and increased the content of high density lipoprotein （HDL） （P%0. 05）. 3）The addition of low-dose resveratrol in basal diet significantly decreased the activity of GSH-Px in rat serum （P%0.05）. Compared with the high fat diet group, the addition of low or high dose of resveratrol in the high fat diet significantly reduced the MDA concentration in serum （P%0.05）, increased T-SOD activity and T-AOC （P%0.05）. 4）Compa
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2017年第11期2216-2224,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家十三五重点研发计划(2016YFD0501209)
湖南省重点研发计划农业领域技术创新项目(2016NK2124)
关键词
白藜芦醇
高脂饲粮
大鼠
血清生化指标
抗氧化力
resveratrol
high fat diet
rats
serum biochemical indexes
antioxidant capacity
