摘要
目的分析不同高脂血症人群血清胆固醇酯转运蛋白水平及其对血浆各脂蛋白间脂质转运的调节作用。方法脂蛋白电泳结合酶显色法分别检测血清各脂蛋白中的甘油三酯含量;酶联免疫吸附试验测定血清胆固醇酯转运蛋白和氧化型低密度脂蛋白水平;分析胆固醇酯转运蛋白与脂质水平间的相关性。结果高甘油三酯组、高胆固醇组、混合组和对照组中胆固醇酯转运蛋白水平分别为1.89±1.32、2.37±1.30、2.33±1.73和1.58±1.00 mg/L,与对照组比较,高甘油三酯组变化无显著性(P>0.05),高胆固醇组(P<0.01)和混合组均升高(P<0.05)。高脂血症人群高密度脂蛋白中胆固醇/甘油三酯比值降低;高甘油三酯组和混合组低密度脂蛋白中甘油三酯/胆固醇比值升高,高胆固醇组无差别,其中高甘油三酯组变化程度最大,混合组次之,高胆固醇组最小;且胆固醇酯转运蛋白水平与上述比值相关。高脂血症人群血清氧化型低密度脂蛋白水平升高,与低密度脂蛋白中甘油三酯/胆固醇比值高度呈正相关。结论高脂血症人群胆固醇酯转运蛋白水平升高,促进了脂蛋白间脂质转运,使高密度脂蛋白和低密度脂蛋白脂质组成发生变化,促进了动脉粥样硬化的发生和发展。
Aim To investigate the possible interaction of cholesteryl ester transfer protein （CETP） level with the lipid transport between the lipoproteins. Methods Triglyceride levels in lipoproteins were determined by agaresis gel electrophoresis with enzyme colouration, plasma CETP and oxidized low-density lipoprotein （ox-LDL） levels were both determined by enzymelinked immunosorbent assay（ELISA）. The correlations between CETP and lipid levels were also studied. Results CETP levels in hypertriglyceridaemla, hypercholesterolaemia, complex hyperlipidaemia and control subjects were 1.89 ± 1.32, 2.37 ± 1.30, 2.33 ± 1.73 and 1.58 ± 1.00 mg/L, respectively, which didn＇t change significantly in hypertriglyceridaemia group （ P〉 0.05 ）, while increased in hypertriglyceridaemia （ P 〈 0.01 ） and hypertriglyceridaemia （ P 〈 0.05 ）. Compared with the control group, the ratios of cholesterol/triglyceride in high density lipoprotein （HDL） decreased in hyperlipidaemia subjects. The ratios of cholesterol/triglyceride in LDL increased both in hypertriglyceridaemia and complex hyperlipidaemia groups, while showed no difference in hypercholesterolaemia group. The two ratios in hypertriglyceridaemic subjects both exhibited the most significant change, while hypercholesterolaemic subjects the least. CETP level had positive correlation with the ratios above. Morever, oxidized LDL level was also found to have positive correlation with LDL-TG/LDLC ratio. Conclusions The high CETP level in hyperlipidaemia subjects promoted the transport of lipids between lipoproteins, which changed the composition of HDL and LDL and was involved in the development of atherescleresis.
出处
《中国动脉硬化杂志》
CAS
CSCD
2006年第12期1038-1040,共3页
Chinese Journal of Arteriosclerosis
基金
国家自然科学基金项目(30471649)
江苏省自然科学基金青年科技创新人才项目(BK2006529)
