摘要
目的探讨槲皮黄酮对酒精性肝损伤大鼠高同型半胱氨酸血症(HHcy)及氧化应激的作用。方法将30只大鼠随机分为正常对照组、模型组、低剂量槲皮黄酮治疗组、中剂量槲皮黄酮治疗组、高剂量槲皮黄酮治疗组,每组6只。用酒精+0.5 mL鱼油灌胃诱导酒精性肝损伤模型,造模3周后,治疗组大鼠分别给予4、2、1 g/kg槲皮黄酮灌胃,7周后麻醉处死大鼠,测定血浆总同型半胱氨酸(tHcy)浓度及血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、白蛋白(Alb)、白/球蛋白比值(A/G)、肝匀浆丙二醛(MDA)、超氧化物歧化酶(SOD)和还原型谷胱甘肽(GSH)表达水平,并对肝脏病理组织进行检测。结果模型组大鼠tHcy、ALT、AST、MDA的表达水平高于正常对照组,而SOD、GSH含量低于对照组,差异均有统计学意义(P<0.05);治疗后,槲皮黄酮治疗组大鼠tHcy、ALT、AST、MDA均低于模型组(P<0.05),而SOD、GSH含量高于模型组(P<0.05),并呈浓度依赖性。组织病理学检测显示,模型大鼠出现肝细胞脂肪变性、点状坏死,伴随炎性细胞浸润,槲皮黄酮治疗后,点状坏死和炎性细胞浸润消失。结论槲皮黄酮可改善酒精性肝损伤,其作用机制可能与降低高同型半胱氨酸水平及氧化应激有关。
Objective To investigate the effects of quercetin on hyperhomocysteinemia and oxidative stress in rats with alcoholic liver injury. Methods Thirty rats were randomly divided into 5 groups： control group,model group,and 3 quercetin treatment（ 4,2 and 1 g/kg） groups. Ethanol plus 0. 5 mL fish oil were used to establish models by in-ducing alcoholic liver injury. At 3 weeks after modeling,4,2 and 1 g/kg of quercetin was given in treatment groups.The plasma total homocysteine（ tHcy）, serum amino transferase activity（ ALT and AST）,albumin（ Alb）,globulin（ Glb）, liver malodialdehyde（ MDA）, seperoxidezed dismutase（ SOD） and glutathione（ GSH） were detected after 7 weeks, and the situation of liver injury was also evaluated by using tissue pathological examination. Results The contents of tHcy,ALT,AST and MDA in model group were higher than those of control group（ P〈0. 05）,while SOD and GSH levels were lower（ P〈0. 05）. After treatment, the contents of tHcy,ALT,AST and MDA in quercetin treatment groups were lower than those of model group（ P〈0. 05）,while the levels of SOD and GSH were higher, and the changes in quercetin treatment groups were in aconcentration-dependent manner. The fatty degeneration,focal necrosis and inflammatory cell infiltration in liver occurred in model group,while the focal necrosis and inflammatory cell infiltration disappeared after treatment with quercetin. Conclusion Quercetin can protect liver against alcoholic liver injury by reducing the tHcy level and oxidative stress.
作者
冯莉芳
张玲莉
FENG Li-fang ZHANG Ling-li(Department of Infectious Diseases Department of Pharmacy, Renmin Hospital of Wuhan University, Wuhan 430060, China)
出处
《实用药物与临床》
CAS
2017年第10期1115-1118,共4页
Practical Pharmacy and Clinical Remedies
