摘要
目的:观察鲜葛花汁对急性酒精中毒大鼠体内乙醇代谢的影响,探讨鲜葛花汁预防醉酒作用的机制。方法:90只大鼠随机分为正常组、模型组、鲜葛花汁大、中、小剂量组和海王金樽组,除正常组外,其余各组大鼠均用白酒灌胃法造模。采用乙醇干片试剂法分别测定末次灌胃后1、2、3、5小时大鼠血中乙醇浓度;用比色法测定大鼠肝、胃组织乙醇脱氢酶(ADH)活性的变化;分别用黄嘌呤氧化酶法、TBA法、比色法检测大鼠肝组织匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)的含量。结果:鲜葛花汁各组大鼠血中乙醇浓度低于模型组,差异有显著性意义(P<0.01);鲜葛花汁各组大鼠肝、胃组织ADH活性高于模型组(P<0.05),鲜葛花汁可显著提高大鼠肝组织SOD、GSH-Px水平,降低MDA水平,与模型组相比差异有显著性意义(P<0.01)。结论:鲜葛花汁通过提高ADH活性,加强乙醇在胃肠道的首过效应,降低血中的乙醇浓度,激活微粒体乙醇氧化系统(MEOS),加速乙醇及其代谢产物的消除速率,达到解酒的目的。
Objective：In order to probe deeply into the mechanism of preventing drunkenness,we observated the alcohol metabolism of acute alcohol poisoning rats feeded by fresh flos puerariae juice.Methods：Ninety mice were allocated to six groups：normal control group,model group,low-dose group,middle-dose group,high-dose group,and positive control group.The mice were filled with white spirit to build alcoholism model;then,we measured the activity of ADH、SOD、MDA and GSH-Px,The concentration of ethanol in blood was detected with chemistry automatic analyzer.Results： Different concentrations of fresh flos puerariae juice could reduce the concentration of ethanol and the content of MDA significantly（P0.01）;the activity of SOD,ADH and GSH-Px were higher than those of model group（P0.05,P0.01）,while the activity of MDA was lower compared with model group and Hai Wangjinzun group（P0.01）.Conclusion： The fresh flos puerariae juice may sober the mice up from the two following aspects：improve ADH activity to reduce concentration of ethanol in blood and enhance the role of first pass metabolism;activate the system of MEOS to accelerate the climination of ethanol and ethanol metabolic production.
出处
《中西医结合肝病杂志》
CAS
2012年第1期38-40,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
武汉市卫生局临床医学科研项目(No.WZ08A01)
关键词
急性酒精中毒
鲜葛花汁/药效学
乙醇代谢
acute alcohol poisoning
fresh flos puerariae juice/pharmacodynamics
alcohol metabolism
