摘要
目的探讨大黄素防止肝纤维化的作用。方法用SD大鼠120只随机分为：(1)假手术+生理盐水组(SO+NS组)；(2)单纯胆总管结扎+生理盐水组(BDL+NS组)；(3)胆总管结扎+大黄素组(BDL+ED组),每组40只。BDL+NS组作胆总管结扎、生理盐水灌胃；BDL+ED组行胆总管结扎、大黄素混悬液灌胃。分别于14、21 d各处死每组大鼠的50%,观察肝纤维化情况和检测肝功能、小肠细菌移位。结果结扎胆总管大鼠的肠黏膜、肝纤维化及肝功能的受损随结扎时间延长而逐渐加重,但用大黄素组有显著减轻,结扎后21 d BDL+NS组与BDL+ED组比较,细菌移位率为(85%比50%,P＜0．05)；肝纤维化程度(按0～4级计)为[0、4、6、8、2比4、10、3、3、0只,(P＜0．01)]；肝功能：ALT[(212．4±19．2)U/L比(146．5±16．2)U/L(P＜0．01)]；AST [(709．6±39．9)U/L比(612．2±30．8)U/L(P＜0．05)]；TB[(174．4±32．2)μmol/L比(159．8±27．2)μmol/L P＜0．05)]。结论大黄素能降低阻塞性黄疸时的肠道细菌移位率,对肝功能有明显保护作用,可有效减轻梗阻性黄疸时的肝纤维化。
Objective To observe the preventive effect of emodin on the liver fibrosis （eirrho6is）. Methods 120 SD rats were randomly divided into three groups： the sham operation group （SO＋ NS group）, choledoehus ligature ＋ normal saline group （BDL ＋ NS group ） and choledochus ligature ＋ emodin group （BDL＋ ED group,40 in each group. Each group was randomly divided into two subgroups： 14-day group and 21-day group, each having 20 rats. After 14 and 21 days, rats were sacri（ieed. Plasm levels o（ ALT, AST and TB were determined, and bacterial translocation rate and degree of liver fibrosis were evaluated too. Results On the 21st day after experiments, bacterial translocation rate was obviously higher in BDL ＋ NS group than in BDL ＋ ED group （85% vs 50%, P〈0.05 ） ;liver fibrosis （ by 0-4 degrees） was more severe in BDL ＋ NS group than in BDL ＋ ED group （0, 4, 6, 8, 2 vs 4, 10, 3, 3, 0 rats, P〈0.01） ; plasm ALT, AST and TB wereremarkably higher in BDL ＋ NS group than in BDL ＋ ED group （ALT： 212.4 ± 19.2 vs 146.5 ± 16.2, P 〈0.01;AST： 709.6 ± 39.9 vs 612.2 ± 30.8, P〈0.05;TB：174.4 ± 32.2 vs 159.8 ±27.2, P〈0.05） .Conclusion Emodin can obviously decrease the degrees of liver fibrosis, decline the plasm levels of ALT, AST and TB and bacterial translocation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第6期681-682,共2页
Chinese Journal of Experimental Surgery
关键词
阻塞性黄疸
大黄素
肝纤维化
细菌移位
Obstructive jaudince
Emodin
Liver fibrosis
Bacterial transloeation
