摘要
目的:探讨鳖甲炮制前后抗肝纤维化活性部位的差异,为鳖甲药材的临床用药提供依据。方法:运用SDS-PAGE技术,对生鳖甲与醋鳖甲活性部位进行分析比较;采用MTT比色法测定生鳖甲与醋鳖甲对HSC-T6增殖的影响。结果:炮制后的醋鳖甲所含成分增多,一级谱带相对分子质量降低,导致小分子肽类物质产生;生鳖甲和醋鳖甲均具有抗肝纤维化的作用,但醋鳖甲的药效优于生鳖甲。结论:鳖甲炮制前后活性部位明显差异,为临床选择用药及进一步活性成分研究提供依据。
Objective： To study the difference in anti-hepatic fiborsis components between pre- and post-processed Trionyx Sinensis Carapace to guide the clinical application of Trionyx Sinensis Carapace. Methods： SDS-PAGE gel electrophoresis was used to compare the constituents in pre- and post-processed Trionyx Sinensis Carapace, and the inhibitory effect on the proliferation of HSC-T6 was determined by MTT. Results： The processed Trionyx Sinensis Carapace had much more components than the crude Trionyx Sinensis Carapace, and the relative molecular mass in the first-level band decreased resulting in the generation of micromolecular polypeptides. Both pre- and post-processed Trionyx Sinensis Carapace had anti-hepatic fibrosis, while the vinegar-processed Trionyx Sinensis was more ef- fective. Conclusion： The difference in the active components in pre- and post-processed Trionyx Sinensis Carapace is obvious, which provides foundation for the clinical application and further researches.
作者
高建蓉
胡祖良
施静妮
唐尹萍
刘焱文
胡春玲
Gao Jianrong Hu Zuliang Shi Jingni Tang Yinping Liu Yanwen Hu Chunling(Zhejiang Quhua Hospital, Zhejiang Quzhou 324004, China Key Laboratory of Chinese Medicine Chemistry and Chinese Herbal Compound of Hubei Province, Hubei University of Traditional Chinese Medicine)
出处
《中国药师》
CAS
2017年第9期1543-1544,1563,共3页
China Pharmacist
基金
浙江省自然科学基金项目(编号:LY14H280001)
湖北省教育厅重点项目(编号:D20162004)
