摘要
目的:研究水药金耳环多糖对实验性2型糖尿病模型大鼠糖脂代谢、肾功能及氧化应激的影响,为金耳环多糖的开发利用提供参考。方法:将60只SD大鼠随机分为正常对照组、模型对照组、阳性对照组(厄贝沙坦片,0.02 g/kg)和金耳环多糖低、中、高剂量组(以生药计分别为2、4、8 g/kg),每组10只。除正常对照组外,其余各组大鼠均ip链脲佐菌素75 mg/(kg·d)复制糖尿病大鼠模型。成模后,各给药组大鼠ig相应药物,正常对照组和模型对照组大鼠ig等体积5%羧甲基纤维素钠溶液,每天2次,连续42d。给药结束后,采用紫外分光光度计检测肝组织中肝糖原含量;采用全自动生化分析仪检测大鼠24 h尿量、24 h尿蛋白含量,血清中三酰甘油(TG)、胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、肌酐(Cr)、尿素氮(BUN)水平,以及肾组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、超氧化物歧化酶(ROS)、丙二醛(MDA)水平。结果:与正常对照组比较,模型对照组大鼠肝组织中肝糖原含量减少(P<0.05);24 h尿量、24 h尿蛋白量增加(P<0.05);血清中TG、TC、LDL-C水平升高(P<0.05),HDL-C水平降低(P<0.05);肾组织中SOD、CAT、GSH-Px水平降低(P<0.05),ROS、MDA水平升高(P<0.05)。与模型组比较,除肝组织中肝糖原含量、血清中HDL-C水平以及金耳环多糖低剂量组大鼠24 h尿量改善不显著外,其余各给药组大鼠上述指标均得到明显改善(P<0.05)。结论:金耳环多糖可调节实验性2型糖尿病模型大鼠的血脂代谢紊乱、改善大鼠肾功能和提高肾抗氧化能力。
OBJECTIVE：To study the effect of Shui medicine Asarum insigne polysaccharide on glycolipid metabolism,renal function and oxidative stress in rats with experimental type 2 diabetes,and provide reference for its development and use. METHODS：60 SD rats were randomly divided into normal control group,model control group,positive control group（Irbesartan tablets,0.02 g/kg） and A. insigne polysaccharide low-dose,medium-dose,high-dose groups（calculated by crude drug as 2,4,8 g/kg）,10 in each group. Except for normal control group,rats in other groups were intraperitoneally injected streptozotocin 75 mg（/kg·d）to induce model with diabetes. After modeling,rats in each administration group were intraperitoneally injected relevant medicines,and rats in normal control group and model control group were intragastrically administrated 5% carboxymet hylcellulose sodium solution,twice a day,for 42 d. After administration,UV spectrophotometer was used to detect the glycogen content in liver tissue. Automatic biochemical analyzer was used to detect 24 h urine output,24 h urinary protein content of rats,levels of triglyceride（TG）,cholesterol（TC）,low-density lipoprotein cholesterol（LDL-C）,high-density lipoprotein cholesterol（HDL-C）,creatinine（Cr）,urea nitrogen（BUN）in serum,and levels of superoxide dismutase（SOD）,glutathione peroxidase（GSH-Px）,catalase（CAT）,superoxide dismutase（ROS）,malondialdehyde（MDA）in kidney tissue. RESULTS：Compared with normal control group,glycogen content in liver tissue of rats in model control group was decreased（P〈0.05）;24 h urine output and 24 h urinary protein content were increased（P〈0.05）;levels of TG,TC,LDL-C in serum were increased（P〈0.05）,and HDL-C level was decreased（P〈0.05）;levels of SOD,CAT,GSH-Px in kidney tissue were decreased（P〈0.05）,and levels of ROS,MDA were increased（P〈0.05）. Compared with model group,except that there were no significant differences in the improvement of uri-nary protein conte
出处
《中国药房》
CAS
北大核心
2017年第31期4415-4419,共5页
China Pharmacy
基金
贵州省黔南州科技局资助项目(No.黔南科合社字[2016]19号)
关键词
水药
金耳环多糖
2型糖尿病
血糖
血脂
肾损伤
氧化应激
大鼠
Shui medicine
Asarum insigne polysaccharide
Type 2 diabetes
Blood glucose
Blood lipid
Renal injury
Oxida-tive stress
Rats
