摘要
目的观察白内障患者房水与血清中白介素-17(IL-17)、基质金属蛋白酶-2(MMP-2)的浓度,探讨其在白内障发病过程中的临床意义。方法选择2016年1月至2017年1月我院收治的白内障患者90例为患者组,选择同期来我院体检的健康志愿者80例为对照组,运用酶联免疫吸附法(ELISA)检测两组受试对象血清与房水中IL-17和MMP-2水平,并对白内障患者血清及房水IL-17和MMP-2水平实施相关性分析。结果患者组治疗前血清IL-17含量为(27.85±3.72)ng/l,明显高于对照组血清IL-17含量(5.03±0.24)ng/l(P<0.05);患者组治疗前血清MMP-2含量为(12.36±2.42)pg/ml,明显高于对照组血清MMP-2含量(3.97±0.35)pg/ml(P<0.05)。患者组治疗前房水IL-17含量为(18.62±2.51)ng/l,明显高于对照组房水IL-17含量(2.12±0.65)ng/l(P<0.05);患者组治疗前房水MMP-2含量为(9.44±1.32)pg/ml,明显高于对照组房水MMP-2含量(1.37±0.25)pg/ml(P<0.05)。经Pearson相关分析显示,患者组白内障患者血清中IL-17与MMP-2水平呈正相关(r=0.736,P<0.05),患者组白内障患者房水中IL-17与MMP-2水平呈正相关(r=0.704,P<0.05)。结论 IL-17和MMP-2在白内障患者房水与血清中存在高表达,其可能跟此病患者眼内炎症反应紧密相关,参与白内障的发生及发展。
Objective To investigate the clinical significance of interleukin-17（IL-17） and matrix metalloproteinase-2（MMP-2） concentration in aqueous humor and serum of patients with cataract.Methods 90 cataract patients who were treated in our hospital between January 2016 and January 2017 were selected,and 80 healthy volunteers were enrolled as control.Enzyme-linked immunosorbent assay（ELISA） was used to detect serum IL-17 and MMP-2 levels and correlation analysis of serum and aqueous humor MMP-2 and IL-17 were conducted.Results Before treatment,both IL-17 and MMP-2 weresignificantly higher in serum and anterior chamber in cataract patients than in normal subjects（all P〈0.05）.Pearson analysis showed that serum and aqueous humor IL-17 levels were positively correlated to corresponding MMP-2 levels in cataract patients（both P〈0.05） Conclusions IL-17 and MMP-2 are highly expressed in the aqueous humor and serum of cataract patients,which may be closely related to the inflammatory reactions in these patients,and both can be actively participated in the occurrence and development of cataract.
出处
《临床眼科杂志》
2017年第5期416-418,共3页
Journal of Clinical Ophthalmology
