摘要
目的探究尼莫地平对蛛网膜下腔出血(SAH)模型大鼠脑干组织中谷氨酸转运体表达情况影响。方法选取54只Wistar大鼠,按照随机数字表法分为对照组6只,SAH组24只和SAH-尼莫地平组24只,使用枕大池二次注血法建立SAH模型,SAH-尼莫地平组注射尼莫地平,分别检测三组大鼠基底动脉的最大血流速度以及谷氨酸转运体(EAATs)m RNA表达水平。结果 SAH组第1、7、14天的基底动脉最大血流速度(Vba)显著高于对照组,SAH-尼莫地平组第1、7天的Vba显著高于对照组,SAH-尼莫地平组在第1、7、14天的Vba显著低于对照组,差异均有统计学意义(P<0.05)。SAH组第1天的EAAT-1/甘油醛-3-磷酸脱氢酶(GAPDH)显著低于对照组,第21天显著高于对照组;SAH-尼莫地平组4个时间点的EAAT-1/GAPDH值均显著高于对照组,第1、7、14天显著高于SAH组,差异均有统计学意义(P<0.05)。SAH组第1、7天的EAAT-2/GAPDH显著低于对照组,SAH-尼莫地平组第14、21天的EAAT-2/GAPDH显著高于对照组,4个时间点均显著高于SAH组,差异均有统计学意义(P<0.05)。SAH组第1、7天的EAAT-3/GAPDH显著低于对照组,SAH-尼莫地平组第1天的EAAT-3/GAPDH值均显著高于对照组,第1、7天显著高于SAH组,差异均有统计学意义(P<0.05)。结论使用尼莫地平可有效增加大鼠脑干中EAATs的表达水平,从而缓解脑血管痉挛,治疗SAH。
Objective To investigate the effects of Nimodipine on the expression of glutamate transporter in the brain stem of subarachnoid hemorrhage（SAH） rats. Methods Fifty-four Wistar rats were randomly divided into control group（n=6）, SAH group（n=24） and SAH-Nimodipine group（n=24）.SAH model was established by injecting blood twice. The level of glutamate transporter（EAATs） m RNA and the maximal blood flow velocity of basilar artery in rats were detected by injection of Nimodipine.Results The Vba of SAH group was significantly higher than that of control group at 1, 7 and 14 d（P〈0.05）. The Vba of SAH-Nimodipine group was significantly higher than that of control group at 1 and 7 d（P〈0.05）. The Vba of 1, 7 and 14 d were significantly lower than those of the control group（ P〈0.05）.EAAT-1/glyceraldehyde-3-phosphate dehydrogenase（GAPDH） of SAH group was significantly lower than that of control group at 1 d（P〈0.05）, and 21 d significantly higher than that of control group（P〈0.05）.The EAAT-1/GAPDH of SAH-Nimodipine group was significantly higher than that of the control group at4 time points（P〈0.05）; the 1, 7 and 14 d were significantly higher than SAH group（P〈0.05）. The EAAT-2/GAPDH of SAH group was significantly lower than that of the control group at 1 and 7 d（ P〈0.05）, and the EAAT-2/GAPDH of SAH group was significantly higher than that of SAH group at 14 d and 21 d after SAH-Nimodipine treatment（P〈0.05）. The EAAT-3/GAPDH of SAH group was significantly lower than that of the control group at 1 and 7 d（P〈0.05）. The EAAT-3/GAPDH of SAH group was significantly higher than that of SAH group at 1 and 7 d after SAH-nimodipine treatment（P〈0.05）.Conclusion Nimodipine can effectively increase the expression of EAATs, thereby relieving cerebral vasospasm, treatment of SAH.
出处
《中华神经创伤外科电子杂志》
2017年第4期226-229,共4页
Chinese Journal Of Neurotraumatic Surgery：Electronic Edition
关键词
尼莫地平
蛛网膜下腔出血
谷氨酸运转体
Nimodipine
Subarachnoid hemorrhage
Excitatory amino acid transporters
