摘要
为探讨N-氨甲酰谷氨酸(NCG)对大菱鲆幼鱼营养组成及免疫功能的影响,以初始体重(24.89±0.51)g的大菱鲆幼鱼为试验对象,在基础饲料中分别添加0(对照组)、0.01%、0.02%、0.04%、0.08%的N-氨甲酰谷氨酸,进行8周养殖。结果表明:NCG对大菱鲆幼鱼全鱼和肌肉营养成分有影响,0.01%组和0.02%组全鱼水分较低,0.02%组全鱼粗蛋白质最高,对照组肌肉水分显著高于其他各组(P<0.05),对照组和0.01%组肌肉粗蛋白质含量显著低于其他各组(P<0.05)随着NCG水平升高,全鱼和肌肉粗脂肪含量逐渐下降,对全鱼灰分影响不显著(P>0.05)NCG水平在0~0.02%间,肌肉中精氨酸和氨基酸总量逐渐升高,继续增加饲料中NCG含量,指标无显著性升高。血清总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)、一氧化氮含量和肝脏中精氨酸酶活性呈现先升高后下降的趋势,各组间指标呈一定的差异。血清中总抗氧化能力(T-AOC)、一氧化氮合酶(NOS)活性逐渐增大,各组间指标呈一定的差异。通过饲料中NCG添加浓度与精氨酸酶活性回归分析,NCG的适宜量为0.0212%。上述研究表明大菱鲆幼鱼饲料中添加适量NCG有助于促进机体内源精氨酸合成,提高肌肉中精氨酸的含量,进而促进蛋白质合成,并可提高机体免疫功能。
This experment was conducted to study the effect of N-carbamoylglutamate（NCG） on nutri- tion composition and immune responses in juvenile turbot. A 8-week study was conducted to examine the growth effects of N-carbamoylglutamate（0, 0.01%, 0.02%, 0.04%, 0.08%）on juvenile turbot [initial body weight （24.89±0.51） g]. The results showed that NCG had significant effects in whole body and muscle of juvenile turbot. Moisture content of whole body in 0.01% and 0.02% NCG were lower than other groups, crude protein of whole body in 0.02% NCG was higher than other groups,The muscle moisture in the control group was significantly higher than that in other groups （P〈0.05）. Crude protein of muscle in the control group and 0.01% NCG were significantly lower than other groups.With increasing levels of dietary NCG, crude lipid in whole body and muscle decreased, while no significant difference was found in ash of whole body. The content of arginine and ami- no acid in muscle increased significantly with increasing dietary NCG level from 0 to 0.02% （P〈 0.05） and then leveled off. The indexs were not significantly increased. Dietary NCG had significant effects on the serum total superoxide dismutase（T- SOD）, catalase（CAT）, total antioxidant capacity（T-AOC）, Nitric oxide synthase（NOS）, nitric oxide （NO） content and arginase in the liver. With increasing levels of dietary NCG, serum T-SOD, CAT, NO and arginase in the liver gradually increased, and then off. The serum T-AOC and NOS increased with increasing levels of dietary NCG. Base on broken-line analysis for arginase against dietary NCG level, the optimum dietary NCG requirement of juvenile turbot was 0.021 2%. All the results demon- strated that optimum dietary NCG can help promote the synthesis of endogenous arginine, improve the concentration of arginine in muscles, improve protein synthesizing and improve the immune function of the body.
作者
尚晓迪
陈春秀
贾磊
王群山
范文涛
赖玉娇
Shang Xiaodi Chen Chunxiu Jia Lei Wang Qunshan Fan Wentao Lai Yujiao
出处
《饲料工业》
北大核心
2017年第18期5-9,共5页
Feed Industry
基金
现代农业产业技术体系专项经费资助[CARS-47-Z1]
天津现代产业技术体系-水产-海水鱼养殖岗位[ITTFRS2017011]
天津市科技计划项目"海珍品高效养殖技术集成与应用"[合同编号:15ZXBFNC00100]
天津市水产局青年科技创新项目[合同编号:J2016-08青]
关键词
N-氨甲酰谷氨酸
大菱鲆
营养组成
免疫功能
N-carbamoylglutamate
Scophthalmus maximus
nutritional composition
immune responses
