摘要
目的:比较不同方法提取蛇鳞草功能成分的抗菌效果。方法:分别用水煎煮、乙醇回流提取、丙酮回流提取、超声辅助的提取方法对蛇鳞草进行提取,采用试管二倍稀释法分别测定提取液对6种常见细菌的最小抑菌浓度。结果:不同提取方法获得的提取液的抑菌效果均有所差异,其中超声方法获得的醇提取液和丙酮提取液抗菌效果好,超声辅助的丙酮提取方法效果最好。结论:超声辅助的丙酮提取方法为蛇鳞草最佳提取方法。
Objective： Objective： To investigate the bacteriostatic activity of the different Pronephrium Triphllum extracts in vitro. Methods： Extracting the active compounds of Pronephrium Triphllum by the method of water extracting, alcohol reflux extraction, acetone reflux, ultrasonic synergistic extraction, and investigating the bacteriostatic activity of 6 kinds of bacteria strains commonly using tube broth methods. Results ： The bacte- riostatic activities were different with different extracting methods, and the bacteriostatic activities of ultrasonic synergistic extraction were higher, acetone reflux was the best. Conclusion：Acetone reflux with ultrasonic syn- ergistic extraction was the best process about bacteriostatic active compounds of Pronephrium Triphllum.
作者
平华
PING Hua(People＇ s Hospital of Jianggan District, Hangzhou, Zhejiang 310016)
出处
《中国中医药科技》
CAS
2017年第5期584-586,共3页
Chinese Journal of Traditional Medical Science and Technology
基金
浙江省医药卫生科技计划项目(2016KYA052)
关键词
蛇鳞草
抑菌活性
最小抑菌浓度
提取方法
Pronephrium Triphllum
bacteriostatic activity
minimum inhibition concentration
extraction method
