摘要
采用微波辅助丙酮/硫酸铵两相盐析体系萃取万寿菊花中的酚类物质及叶黄素。通过单因素实验,考察了微波作用时间、微波功率、料液比对目标产物在两相间分配行为的影响,确定最优萃取条件,并采用响应曲面法优化微波辅助盐析萃取万寿菊花中多酚的提取工艺。结果表明,多酚、类黄酮及叶黄素富集于上相丙酮相,得率分别为85.76、6.70、0.85 mg/g;回收率分别为96.64%、98.15%、95.98%。响应曲面法优化微波辅助盐析萃取万寿菊花中多酚的最优提取工艺为:微波功率500 W,微波作用时间95 s,料液比(g/m L)为1∶45,粉碎目数160目,在该条件下,多酚得率为86.24 mg/g,并对提取物抗氧化活性作了初步测试,结果表明,提取物对DPPH·及ABTS·+有明显的清除效果,其抗氧化活性优于两相盐析提取物。
Microwave-assisted salting-out extraction of phenolic compounds and lutein from marigold flowers were studied using a system composed of acetone and ammonium sulfate. The effects of microwave action time,microwave power and solid-liquid ratio on the partition behavior of the target products were investigated by single factor experiments,and the extraction condition for phenols in microwave assisted salting-out systems was also optimized by response surface analysis. The results showed that the components were extracted into the top acetone-rich phase,and the extraction rates of total phenolics（ TP）,total flavonoids（ TF） and lutein were 85. 76,6. 70 and 0. 85 mg/g,respectively.The corresponding recoveries were 96. 64%,98. 15% and 95. 98%. The extraction rate of total phenolics reached 86. 24 mg/g under the optimum extraction conditions of microwave power 500 W,microwave irradiation time 95 s,solid-liquid ratio 1 ∶ 45 g/m L and crushing mesh number of marigold160 mesh. In addition,the antioxidant activity of the extracts was preliminarily tested. As a result,the extracts by microwave assisted salting-out extraction could obviously remove DPPH · and ABTS ·＋.Moreover,the antioxidant activity was better than that by salting out two-phase extraction.
作者
马娜
付晓茜
孙伟鹏
党艳艳
MA Na FU Xiao-qian SUN Wei-peng DANG Yan-yan(School of Chemistry and Chemical Engineering, Shihezi University, Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi 832003,Xinjiang, Chin)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2017年第10期1139-1147,共9页
Fine Chemicals
基金
新疆兵团高新领域项目(2015AB007)
石河子大学高层次人才资助项目(RCZX201413)~~
关键词
微波辅助
响应面法
多酚
类黄酮
万寿菊
中药现代化技术
microwave assisted extraction
response surface method
phenols
flavonoid
marigold
modernization technology of traditional Chinese medicines
