摘要
调查了安徽茶区茶树丛枝菌根真菌(AMF)资源分布情况,为菌根生物技术在茶产业中的应用提供了具有应用价值的菌种资源.采用醋酸-墨水染色法观察茶树丛枝菌根(AM)的侵染率、侵染级数、侵染强度和菌根类型;采用湿筛法获得AMF孢子,进行形态学鉴定.结果表明:在安徽茶区,AMF能够侵入茶树根系形成典型的共生体,侵染率在36%~95%,侵染级数均在3级以上,侵染强度大;从安徽茶树根际土中共鉴定出8属36种AMF,其中缩管柄囊霉是优势种,网状球囊霉、刺无梗囊霉、孔窝无梗囊霉、詹氏无梗囊霉、双网无梗囊霉和凹坑无梗囊霉是常见种,褐色管柄囊霉、疣突管柄囊霉、毛氏无梗囊霉、近明管柄囊霉、瑞氏无梗囊霉、空洞无梗囊霉、晕环球囊霉、细齿无梗囊霉、地管柄囊霉、幼套球囊霉、蜜色无梗囊霉、稀有内养囊霉是稀有种,其余17种为少见种;不同采样地茶树根际AMF群落相似性系数(0.14~0.55)较低,多属于低和中等水平;相关性分析表明,孢子密度与侵染率呈显著正相关,种的丰度与侵染率和总球囊霉素相关土壤蛋白呈显著正相关.安徽茶区茶树根系存在典型的AM结构,其根际AMF多样性丰富,为开发茶树专用AMF肥料提供了丰富的菌种资源.
In order to provide tea industry, the distribution potentially valuable resource for of AMF resources from Anhui applying mycorrhizal biotechnology in tea area was studied. AMF infection rate, the series of infection, the intensity of infection and the type of mycorrhizae of tea plant were measured using an improved acetic acid ink staining method. AMF spores were isolated by wet screening technique and identified based on morphological characteristics. The results indicated that AMF could invade the root of tea plant and form AMF-plant symbioses, AMF infection rate ranged from 36% to 95%, and the series of infection was above 3 and the intensity of infection was strong. A total of 36 species of AMF belonging to 8 genera in the rhizosphere of tea plant were identified from Anhui tea area. Among them, Funneliformis constrictum was the dominant species; Glomus reticulatum, Acaulospora spinosa, Acaulospora foveata, Acaulospora gerdemannii, Acaulospora bireticulata and Acaulospora excavata were the common species ; F. badium, F. verruculosum, Acaulospora morrowae , F. claroideum , Acaulospora rehmii , Acaulospora cavernata , G. halonatum , Acaulospora denticulata, F. geosporum, G. etunicatum, Acaulospora mellea and Entrophospor infrequens were the rare species, and the other species were the uncommon species. The communities of AMF shared relatively low similarity （0.14-0.55） at different sampling sites which belonged to the low and medium level. Correlation analysis showed that spore density had a positive correlation with the infection rate, and species richness had a positive correlation with the infection rate and T-GRSP. There was typical AM structure in root of tea plant in Anhui tea area and the diversity of AMF was rich. All of these could provide rich resources for the development of special AMF fertilizer for tea plant.
出处
《应用生态学报》
CAS
CSCD
北大核心
2017年第9期2897-2906,共10页
Chinese Journal of Applied Ecology
基金
国家自然科学基金项目(31400589)
安徽高校自然科学研究重点项目(KJ2012A129)资助~~
关键词
丛枝菌根真菌
孢子
多样性
种类分布
优势种
arbuscular mycorrhizal fungi （AMF）
spore
diversity
species distribution
dominant species
