摘要
为探究干旱胁迫下丛枝菌根真菌(arbuscular mycorrhizal fungi,AMF)对茶树(Camellia sinensis)光合特性的影响,于温室盆栽条件下,对‘福鼎大白茶’实生苗进行水分和接种AMF试验。结果显示,干旱胁迫(田间最大持水量的55%)显著抑制了AMF对茶树根系的侵染,降低了叶片中叶绿素b含量、最大光量子效应(QY_max)值、胞间CO_(2)浓度(Ci)、气孔纵径和横径以及气孔开度,碳同化相关酶基因CsRbcL、CsTK、CsFBPase、CsPRK以及叶绿素合成酶基因CsHEME的表达下调;提高了叶绿素a/b、非光化学猝灭(NPQ_lss)参数,叶绿素合成酶CsHEMA1表达上调。正常供水(田间最大持水量的75%)和干旱胁迫条件下接种AMF,显著提高了叶绿素a和总叶绿素的含量、净光合速率(Pn)、气孔导度(Gs)、Ci、蒸腾速率(Tr),降低了NPQ_lss及气孔密度,不同程度地诱导了两种水分条件下碳同化相关酶基因CsRbcL、CsTK、CsFBPase、CsPRK以及叶绿素合成相关酶基因CsHEMA1、CsHEMC、CsHEME、CsHEMG和CsCHLE的表达。与此同时,接种AMF还显著增加了干旱胁迫下叶绿素b的含量、QY_max、气孔纵经、横径和气孔开度以及正常供水下叶绿素a/b。研究表明,接种AMF可促进茶树的光合作用,提高茶树对干旱的适应性,且在干旱胁迫下效果显著。
In order to explore the effect of arbuscular mycorrhizal fungi(AMF)on the photosynthetic characteristics of tea plants under drought stress,the seedlings of Camellia sinensis.‘Fuding Dabaicha’were subjected to water and AMF inoculation experiments under greenhouse pot conditions.The results showed that drought stress(55%of the maximum field water capacity)significantly inhibited the AMF infection on tea plant roots,reduced the chlorophyll b content,maximum quantum effect(QY_max)value,intercellular CO_(2) concentration(Ci),stomatal vertical and horizontal diameter and stomatal opening,as well as down-regulated the expressions of carbon assimilation-related enzymes(CsRbcL,CsTK,CsFBPase,CsPRK)genes and chlorophyll synthesis-related enzyme gene CsHEME.Whereas,drought stress significantly increased the chlorophyll a/b and non-photochemical quenching(NPQ_lss)parameters,and up-regulated the expression of chlorophyll synthase enzymes gene CsHEMA1 in tea seedlings.Howbeit,under well-watered(75%of the maximum field water capacity)and drought stress conditions,inoculation with AMF significantly increased the content of chlorophyll a and total chlorophyll,net photosynthetic rate(Pn),stomatal conductance(Gs),Ci,and transpiration rate(Tr)but decreased NPQ_lss and stomatal density,accompany with the up-regulated expressions of carbon assimilation-related enzyme genes CsRbcL,CsTK,CsFBPase,CsPRK and chlorophyll synthesis-related enzyme genes CsHEMA1,CsHEMC,CsHEME,CsHEMG and CsCHLE in different degrees.Meanwhile,AMF inoculation also significantly increased the chlorophyll b content,QY_max,stomatal vertical,horizontal diameter and aperture under drought stress and chlorophyll a/b under well-watered.The results indicated that AMF can improve the adaptability of tea plant under drought stress by promoting photosynthesis,and the promotion effect was more significant under drought stress.
作者
陈鑫
邬晓龙
刘升锐
胡贤春
刘春艳
CHEN Xin;WU Xiaolong;LIU Shengrui;HU Xianchun;LIU Chunyan(College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,China;Anhui Agricultural University,State Key Laboratory of Tea Plant Biology and Utilization,Hefei 230036,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2024年第10期2358-2370,共13页
Acta Horticulturae Sinica
基金
安徽农业大学茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20200122)。
关键词
茶树
丛枝菌根真菌
干旱胁迫
气孔
光合特性
tea plant
arbuscular mycorrhizal fungi
drought stress
stomatal
photosynthetic characteristics
