摘要
为了探寻欧洲白桦变种裂叶桦的叶缘分裂机制。以2种桦树为研究对象,在叶片形态特征和解剖特征观测的基础上,进行了顶芽及叶片叶尖、裂片及凹缺等不同组织部位相关基因表达定量分析、内源激素IAA含量测定以及IAA活体标记的GUS染色分析。结果表明,裂叶桦叶片两侧叶缘自叶基到叶尖分裂出由大到小3~4个裂片,叶背面的叶脉呈明显的管状隆起,叶宽、平均二级脉间距、主脉横切面积等分别较欧洲白桦大了21.12%、12.64%、21.46%。BpmiR164s及靶基因的组织表达特性分析结果证明,BpmiR164-2及靶基因BpCUC2与裂片的形成关系密切,是裂叶性状产生的主要调控基因。生长素输出载体BpPINs基因的组织表达特性分析结果证明,6个基因中只有BpPIN2和BpPIN5在裂叶桦的叶尖、裂片及顶芽等组织器官中呈现上调表达。内源激素IAA的活体标记发现,在顶芽、幼叶中IAA的含量较高,而在叶片中,叶尖、叶齿及裂片处是激素的高表达部位,利用液相色谱—质谱联用仪(LC-MS)对2种桦树的上述不同组织部位的生长素测定也证明了激素的上述分布特点。综合上述,裂叶桦裂片产生是激素在叶脉、叶缘的极性运输及相关基因调控的结果。
In order to explore the mechanism of marginal cleavage in Betula pendula ‘Dalecarlica ＇,the research took Betula pendula ‘Dalecarlica ＇ and Betula pendula as research projects,based on leaf morphological characters and anatomical feature,we conducted qRT-PCR of leaf cleavage relating genes in apical buds,leaf tips,lobes,sinuses and other tissues,and also measured the endogenous IAA content and analyzed the GUS dyeing of IAA living tag（ DR5）. It turned out that Betula pendula‘Dalecarlica＇blades owned three or four lobes from base to tip in both leaf sides,the veins on the back showed obvious tubular uplift,leaf width,mean distance between secondary veins,cross sectional areas of main veins were respectively bigger, namely 21. 12%,12. 64%,21. 46%. Tissue expression characteristic analysis of BpmiR164 s and target genes demonstrated that BpmiR164-2 and BpCUC2 played an important role in the formation of lobes,which was the major regulating gene in producing lobes;tissue expression characteristic analysis of BpPINs indicated that only BpPIN2 and BpPIN5 in six auxin efflux carriergenes presented up-regulated expression; living tag of endogenous IAA manifested that IAA content enriched in apical buds and spires,meanwhile,the tissues of leaf tips,teeth and lobes in leaf expressed more IAA,we also got the IAA distribution features from results of LC-MS in above-mentioned tissues. Above all,the formation of lobes was bound up with polar transportation of hormone in the veins and some related gene regulation.
作者
渠畅
边秀艳
姜静
陈肃
刘桂丰
QU Chang BIAN Xiu-yan JIANG Jing CHEN Su LIU Gui-feng(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin,Heilongjiang,150040,P. R. China.)
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2017年第8期9-16,共8页
Journal of Beijing Forestry University
基金
国家自然科学基金项目(31370660)
