摘要
目的:观察模拟微重力下骨碎补对成骨细胞破骨细胞共培养中成骨细胞分化影响及ALP、OPG、Runx2表达作用机制。方法:建立成骨细胞破骨细胞共培养系统,模拟微重力下加骨碎补总黄酮。正常重力组(A组)和微重力空白组(B组)加等容生理盐水组大鼠血清。骨碎补低剂量组(C组)加含低浓度骨碎补血清0.054 g/(kg·d),骨碎补中剂量组(D组)加含中浓度骨碎补血清0.162 g/(kg·d),骨碎补剂量组(E组)加入高浓度骨碎补血清0.486 g/(kg·d)。A组置于培养箱中,B、C、D、E置于摸拟微重力回转器48 h。测成骨细胞ALP活性、OPG、Runx2表达。结果:与正常对照组比较,微重力组成骨细胞ALP活性降低(P<0.05);与微重力空白对照组比较,含药组ALP活性、OPG和Runx2表达升高,中剂量组差异显著(P<0.05)。结论:中剂量骨碎补能提高微重力下成骨细胞破骨细胞共培养系统中提高成骨细胞ALP活性、OPG、Runx2表达,促成骨细胞增殖、分化。
Objective： To observe the effect of TFDF on the differentiation of osteoblasts in osteoblasts/osteoclasts co-culturing under microgravity,to explore its mechanism on the expressions of ALP,OPG,and Runx2. Methods： Osteoblasts/osteoclasts were incubated in the same culturing condition,TFDF was given under microgravity. There were five groups involved in this research as follows： the normal gravity group（ group A）,the microgravity group（ group B）,low-dose TFDF group（ group C）,medium-dose TFDF group（ group D）,and high-dose TFDF group（ group E）. Rats serum was added to group A and group B,which were given 0. 9% Nacl. Rats serum was added to group C at 0. 054 g/kg,at 0. 162 g/kg in group D,and at 0. 486 g/kg in group E. Placed group A in normal gravity,groups B,C,D and E were placed under microgravity gyrator and cltured for 48 hours. ALP,OPG and Runx2 expression were measured.Results： Compared to group A,ALP was decreased in group B（ P〈0. 05）; compared to group B,ALP,OPG and Runx2 expression were elevated in the TFDF intervention groups,in which group D had significant difference（ P〈0. 05）. Conclusion： The medium-dose TFDF can increase LP,OPG,and Runx2 expression,promote growth and differentiation of the osteoblasts/osteoclasts under microgravity.
作者
尹文哲
张小玲
叶义杰
田鑫铎
YIN Wen- zhe ZHANG Xiao- ling YE Yi -jie TIAN Xin -duo(The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China)
出处
《中医药学报》
CAS
2017年第4期16-20,共5页
Acta Chinese Medicine and Pharmacology
基金
黑龙江省中医药科研项目(ZHY12-Z160)
