摘要
目的观察回转器模拟失重条件下骨形态发生蛋白 2 (BMP 2 )对大鼠骨肉瘤成骨样细胞 (ROS1 7/ 2 .8)基因表达的影响。方法在 1G和回转器模拟失重条件下对ROS1 7/ 2 .8细胞进行培养 ,培养液中含5 0 0ng/ml的BMP 2。在实验的 2 4、48和 72h提取细胞总RNA ,进行反转录PCR ,检测Ⅰ型胶原α1链(COL Ⅰα1 )及碱性磷酸酶 (ALP)mRNA的表达 ,并计算与内参照 β -肌动蛋白 ( β actin)mRNA水平的比值。结果BMP 2诱导组的COL Ⅰα1mRNA表达在 2 4h和 48h显著高于无BMP 2组 (P <0 .0 1 ) ,ALPmRNA水平在 48h和 72h显著高于无BMP 2组 (分别为P <0 .0 1 ,P <0 .0 5 ) ;BMP 2诱导 48h后模拟失重组的COL Ⅰα1mRNA水平显著低于 1G对照组 (P <0 .0 1 ) ,模拟失重 48h和 72h后ALPmRNA表达显著低于 1G对照组 (P <0 .0 1 )。结论BMP 2可促进大鼠骨肉瘤成骨样细胞的分化相关基因的表达 ,在模拟失重条件下细胞对BMP 2的促分化作用的反应性下降。
Objective To investigate the effect of BMP-2 on (the) gene expression of rat osteosarcoma cells (ROS17/2.8) under rotating clinostat simulated weightlessness. Mothod ROS17/2.8 cells were cultured in 1 G control and rotating clinostat simulated weightlessness with 500 ng/ml BMP-2 in the culture medium. Total RNA in cells was isolated after 24, 48 and 72 h. Reverse transcription PCR analysis was made to examine the gene expression of α1 chain of type I collagen (collagen Ⅰ α1) and alkaline phosphatase (ALP). Result The expression of COL-Ⅰα1 mRNA induced by BMP-2 was much more than that without BMP-2 in 24 h and 48 h group(P<0.01). The level of ALP mRNA induced by BMP-2 was significantly high in 48 h or 72 h group(respectively P< 0.01,P< 0.05). The level of BMP-2 induced expression of COL-Ⅰα1 mRNA was significantly lower under 48 h of simulated weightlessness than in 1G condition( P<0.01). The level of ALP mRNA was significantly lower under simulated weightlessness for 48 h or 72 h(P<0.01). Conclusion BMP-2 can stimulate the differentiation of ROS17/2.8 cells in 1 G condition and this process is reduced under simulated weightlessness.
出处
《航天医学与医学工程》
CAS
CSCD
北大核心
2004年第3期176-179,共4页
Space Medicine & Medical Engineering
