摘要
目的 :用酶消化法培养Balb/c胎鼠下颌突外胚间充质细胞 ,并与组织块法相比较 ,研究其形态和生长特性。方法 :采用 1.2 5 g/L的胰酶消化法获取E 12 .5dBalb/c胎鼠下颌突外胚间充质细胞 ,用含 10 6U/LLIF的D/F12 培养细胞 ,倒置显微镜下观察细胞形态和生长情况 ,免疫组化鉴定细胞来源及分化状况。结果 :培养的细胞呈单层生长 ,长梭形 ,有 2～ 4个突起 ,抗HNK - 1、Vimentin、S - 10 0、NSE染色阳性 ,抗GFAP、NF、MA染色阴性 ,证实为未分化外胚间充质细胞。结论 :用酶消化法可在短期内获得大量的未分化外胚间充质细胞。
AIM:Primary ectomesenchymal cells of Balb/c fetal mandibular process were got by trypsin. Then their morphology and growth rate was studied. METHODS: The primary ectomesenchymal cells of E 12.5 Balb/c fetal mice mandibular process were isolated by 1.25 g/L trypsin and cultured with D/F 12 with 10 6U/L LIF. Their morphology and growth rate were observed by phase-contrast microscope. Immunohistochemistry was used to identify the characteristics of cells. RESULTS: The cultured cells displayed monolayer growth and were fibroblast-like with 2～4 processes. Cells were anti-HNK-1?Vimentin?S-100?NSE positive and anti-GFAP?NF?MA negative in immunohistochemistry. CONCLUSION: A large number of undifferentiated ectomesenchymal cells could be obtained by 1.25 g/L trypsin in a short time.
出处
《牙体牙髓牙周病学杂志》
CAS
2002年第8期403-406,I001,共5页
Chinese Journal of Conservative Dentistry
