摘要
目的了解江苏地区流通环节双壳贝类中诺如病毒(Norovirus,NV)携带和流行状况,为预防食源性疾病的发生和食品安全风险评估提供基础资料。方法 2015年7月—2016年6月,从江苏6个市(县)的流通和养殖环节采集657份贝类海产品样本,采用RT-PCR法检测NV。结果共采集657份标本,监测标本NV阳性率为15.22%,以GⅡ型为主(13.39%),其次为GⅠ型(6.85%)、GⅠ+GⅡ型NV同时检出(5.02%),不同亚型NV阳性率差异有统计学意义(χ~2=11.389,P<0.05)。不同双壳贝类NV检出率差异有统计学意义(χ~2=67.677,P<0.05),牡蛎中NV感染率最高(24.83%);不同地区样品阳性率的差异有统计学意义(χ~2=21.67,P<0.05),东台阳性率最高(23.64%)。不同季度双壳贝类阳性率的差异有统计学意义(χ~2=9.518,P<0.05),2016年第一季度阳性率最高(18.16%)。结论牡蛎为携带NV的高危食品,沿海城市中销售的双壳贝类感染水平较高,NV携带存在季节性的变化。
Objective To explore the epidemic status and infection situation of Norovirus（NV） in bivalve mollusks in Jiang su provinee; to provide basle information for foodborne diseases prevention and food safety risk assessment. Methods A total of 657 shellfish seafood samples were collected in the circulation and culture links from six cities ineluding Wuxi, Jiangyin, Nanjing, Qidong, Rudong, Dongtai in Jiangsu province from Jul 2015 and Jun 2016, RT-PCR was used to detect NV infection. Results A total of 657 samples eollected in the survey, the NV positive rate of above surveyed samples was 15.22%. GII type was the main type （13.39%）, followed by GI （6.85%） and GI＋GII dural infection（5.02%）; the infection rates of different subtypes were statistically significant （χ^2= 11. 389, P〈0.05）; the infection rates of different bivalves were statistieally significant （χ^2= 67. 677,P〈0. 05）,the highest NV infection rate was found among oyster samples （24. 83 %）. The infeetion rates of samples from different cities were statistically significant （X2 = 21.67, P〈0.05）, the highest NV infeetion rate was found among samples eolleeted in Dongtai （23.64%）. The NV infection rates of bivalves in 4 seasons were statistieally significant （2： 9. 518,P〈0. 05）, the highest infection rate was found among samples eollected in the first quarter of 2016 （18. 16%）. Conclusion Oysters were high risk food for NV infection. The NV infection rate of bivalves sold in eoastal cities was relatively high, with seasonal fluctuation.
出处
《江苏预防医学》
CAS
2017年第4期395-397,402,共4页
Jiangsu Journal of Preventive Medicine
基金
南通市卫计委青年科研基金(WQ2016049)
