摘要
为建立一种快速、特异、敏感的鸡奇异变形杆菌检测方法。本研究针对鸡奇异变形杆菌全基因序列设计了1对特异性引物,建立检测鸡奇异变形杆菌的SYBR GreenⅠLC-PCR检测方法,进行敏感性、特异性、重复性试验,并通过临床组织样品检测评价其临床实用性。结果显示,所建立方法的标准曲线线性关系良好,相关系数R^2=0.9957。该方法灵敏度高,可检测到稀释度为10~1 CFU/m L的奇异变形杆菌。特异性强,不与其他禽源致病原发生交叉反应。重复性试验变异系数小于2%。提示该方法可用于鸡奇异变形杆菌的监测和早期临床诊断。
For establishment of a rapid,specific and sensitive detection method for Proteus mirabilis in chicken,we designed a pair of specific primers for r DNA sequence of the bacteria,established the SYBR GreenⅠLC-PCR,tested its sensitivity,specificity and repeatability,and evaluated its clinical utility by detecting the clinical tissue samples.The standard curve revealed a good linear relationship with the correlation coefficient R^2=0.995 7.The method showed high sensitivity,with the minimum detected dilution of 10^1CFU/mL for Proteus mirabilis,high specificity,no cross-reactons with other avian pathogens,and well repeatability with the coefficient of variation less than 2%.The results suggest the established technique could be used for monitoring and early diagnosis of Proteus mirabilis in chicken.
作者
史瑞雅
刘建钗
刘彦威
刘娜
张欣
李博
SHI Rui-ya LIU Jian-chai LIU Yan-wei LIU Na ZHANG Xin LI Bo(School of Life Science and Food Engineering,Hebei University of Engineering,Handan 056021, China Engineering Research Center of Hebei Province of Poultry Disease, Handan 056021, China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2017年第7期856-860,共5页
Chinese Veterinary Science
基金
河北省科技厅项目(2016D15201)
关键词
鸡
奇异变形杆菌
荧光定量PCR
检测
chicken
Proteus mirabilis
SYBR GreenⅠ fluorescence quantitative PCR
detection
