摘要
采用全自动微生物鉴定系统（VITEK-32）鉴定了一例临床分离鸡致病菌,并进行了超广谱β-内酰胺酶（ES-BLs）的检测和用肉汤微量稀释法测定12种药物和4种复方药物对分离菌的抗菌活性。结果显示,分离的4株菌中鉴定为大肠埃希氏菌3株、奇异变形杆菌1株,在分离的4株菌中均产ESBLs。药敏试验结果表明4株菌对氨苄西林、头孢噻肟、头孢噻呋、头孢曲松除一株中介外其它均耐药,对阿米卡星均敏感,尤其1株奇异变形杆菌和1株大肠杆菌耐药严重,对12种药物中有10种耐药,显示出严重的多重耐药性。β-内酰胺类药物与酶抑制剂联用能使药物对细菌的最低抑菌浓度（M ICs）降低4～64倍,因此复方β-内酰胺类药物仍是防治鸡致病菌感染的有效措施之一。
The bacterial strains were isolated from chicken and identified by the VITEK-32 system.The extended-spectrum β-lactamases（ESBLs） were detected by disk diffusion method and antimicrobial susceptibility testing was performed by broth micro-dilution assay using 12 kinds of drugs and 4 kinds of compound drugs.The results revealed that the bacterial strains were identified as Escherichia coli and Proteus mirabilis.The 4 clinically isolated strains could produce ESBLs.The results of antimicrobial susceptibility testing showed that the 3 Escherichia coli strains and 1 Proteus mirabilis strain were all resistant to ampicillin, cefotaxime, ceftiofur and ceftriaxone except 1 Proteus mirabilis strain was intermediate to cefotaxime ; all strains were susceptible to amikaxin ; and 1 Escherichia coli strain and 1 Proteus mirabilis strain isolated from the same diseased chicken were resistant to 10 of 12 kinds of drugs especially. This result demonstrated the serious multi - drug resistance. The minimum inhibitory concentration （MICs） of β- lactam antibiotics combined with enzyme inhibitor against Escherichia coli strains and Proteus mirabilis decreased by 4 - 32 and 8 ～ 64 times, respectively. Thus the application of compoundβ- lactam drugs is one of effective measures for the prevention and treatment of chickens infected with the bacteriosis.
出处
《江西农业学报》
CAS
2009年第12期156-159,共4页
Acta Agriculturae Jiangxi
基金
国家自然科学基金项目(30771624)
