摘要
目的研究胃部非癌组织、胃部癌旁组织与胃部癌组织中自噬基因Beclin1和微管相关蛋白轻链3(microtubule-associated protein 1 light chain 3,MAPLC3)基因的表达含量,确定Beclin1和MAPLC3表达含量与胃癌之间的联系。方法使用实时逆转录-聚合酶链反应(Real-Time reverse transcription polymerase chain reaction,Real-Time RTPCR)检测胃部非癌组织、胃部癌旁组织与胃部癌组织中Beclin1以及MAPLC3基因的mRNA表达含量。使用蛋白质印迹法(Western blot)方法检测胃部非癌组织、胃部癌旁组织与胃部癌组织中Beclin1以及MAPLC3基因的蛋白表达含量。结果与胃部非癌组织相比,胃部癌旁组织Beclin1以及MAPLC3的mRNA含量显著降低(P<0.01),胃部癌组织中Beclin1以及MAPLC3的mRNA含量也显著降低(P<0.01);与胃部癌旁组织相比,胃部癌组织中Beclin1以及MAPLC3的mRNA含量也显著降低(P<0.05)。与胃部非癌组织相比,胃部癌旁组织Beclin1以及MAPLC3的蛋白含量显著降低(P<0.05),胃部癌组织中Beclin1以及MAPLC3的蛋白含量也显著降低(P<0.01);与胃部癌旁组织相比,胃部癌组织中Beclin1以及MAPLC3的蛋白含量也显著降低(P<0.05)。结论自噬相关基因Beclin1以及MAPLC3的mRNA和蛋白水平在胃癌组织中表达降低,这提示自噬相关基因与胃癌的发生、发展密切相关。
Objective： To study the non stomach cancer tissue, autophagy gene Beclinl and microtubule stomach tissues and gastric cancer associated protein light chain 3 （microtubule-associated protein 1 light chain 3, MAPLC3） expression lev- els of genes, and determine Beclinl and MAPLC3 expression between gastric cancer and content contact. Methods： The real- time reverse transcription polymerase （Real-Time reverse transcription poly chain reaction Merase chain reaction, Real-Time RT-PCR） was used to detect gastric non cancer tissues, Beclinl stomach tissues and the stomach cancer tissue and MAPLC3 gene mRNA expression content. Using Western blotting （ Western blot） method was used for detection of gastric non cancer tissue the expression of Beclinl of gastric tissues and gastric cancer tissues, MAPLC3 protein content. Results： Compared with non stomach cancer, the mRNA contents of stomach cancer tissues and Beclinl MAPLC3 were significantly decreased （P 〈 0.01 ）, stomach carcinoma Bechnl and MAPLC3 content of mRNA was lower （P 〈 0.01 ） ; and stomach carcinoma, stomach carcinoma Beclinl and MAPLC3 content of mRNA was lower （P 〈 0.05）. Compared with the non stomach cancer tissue, stomach cancer adjacent tissue Beclinl and MAPLC3 protein content was significantly reduced （P 〈 0.05）, gastric carcinoma Beclinl and MAPLC3 protein content also decreased significantly （P 〈0.01 ） ; Compared to noncancerous tissues, gastric carcinoma Beclinl and MAPLC3 protein content also decreased significantly （P 〈 0.05 ）. Conclusion： The autophagy related gene Beclinl and MAPLC3 mRNA and protein expression decrease in gastric cancer tissues, suggesting that autophagy related genes are closely related with gastric carcinogenesis and development.
出处
《泰山医学院学报》
CAS
2017年第5期484-487,共4页
Journal of Taishan Medical College
关键词
自噬基因
BECLIN1
微管相关蛋白轻链3
胃癌
autophagy gene
Beclinl
microtubule associated protein light chain 3
gastric cancer
