摘要
目的观察饥饿模型大鼠心肌细胞中微管相关蛋白1轻链3(LC3)及自噬基因Beclin 1的表达变化,探讨自噬机制在饥饿大鼠心肌中的作用。方法将42只健康雄性SD大鼠随机分为正常对照组(n=6)和饥饿模型组(n=36)。正常对照组正常饮食,饥饿模型组禁食但自由摄水,根据饥饿天数又分为1、2、3、5、7、9 d等6个亚组(n=6)。各亚组在相应时间进行心脏标本提取并留存,采用免疫组化法和蛋白质印迹法检测心肌自噬蛋白Beclin 1和LC3水平变化。结果与正常对照组相比,饥饿模型组各时间点SD大鼠心肌的自噬蛋白LC3和Beclin 1阳性细胞数及蛋白表达都有不同程度的增加,差异有统计学意义(P<0.01或P<0.05),饥饿模型各亚组之间自噬蛋白LC3和Beclin 1阳性细胞数及蛋白表达差异亦均有统计学意义(P<0.01或P<0.05),且在饥饿2 d和7 d出现2个峰值。结论饥饿状态可通过激活心肌自噬机制参与心肌的病理改变过程。
Objective To observe the expression of microtubule associated protein 1 （LC3） and autophagy gene Bec-lin 1 in rat cardiac myocytes, and to investigate the effect of autophagy on the myocardium of starved rats.Methods 42 healthy male SD rats were randomly divided into normal control group （ n =6） and starvation model group （ n =36）.Normal control group with normal diet, starvation model group were only with free water intake, according to the number of days of the starvation, they were divided into 1d, 2d, 3d, 5d, 7d, 9 d subgroups （ n =6）.The cardiac specimens were extracted and retained, and the levels of autophagy protein LC3 and Beclin1 were detected by immunohistochemistry and Western blotting. Results Compared to normal control group, starvation model group’ s SD rat cardiac myocyte autophagy protein LC3 and Be-clin 1 positive cell number and protein expression increased in different degree, and the differences were statistically signifi-cant （ P 〈0.01 or P 〈0.05）, and there were statistically significant differences of autophagy protein LC3 and Beclin 1 posi-tive cell number and protein expression between different subgroup of starvation model group （ P 〈0.01 or P 〈0.05）, and there were two peaks at 2d and 7 d.Conclusion Starvation involved in the mechanism of myocardial pathological changes of myocardial process by activation of autophagy.
出处
《疑难病杂志》
CAS
2015年第7期719-722,F0003,共5页
Chinese Journal of Difficult and Complicated Cases
关键词
自噬基因
BECLIN
1
微管相关蛋白1轻链
心肌
饥饿
大鼠
Autophagy gene
Beclin 1
Microtubule associated protein 1 light chain
Myocardium
Starvation
Rats
