摘要
目的研究基因Beclin1、UCH-L5、LC3在急性髓系白血病患者体内表达情况及作用。方法选取AML患者80例,进行危险度分层,将患者分组为初发组40例,提起骨髓液,缓解组40例,经化学诱导和药物治疗达到完全缓解后提取骨髓液,选取健康组40例,对比3种基因在不同危险度分层和健康组、初发组、完全缓解组之间的表达差异。对比不同危险度之间自噬相关基因的mRNA表达差异。分别采用自噬抑制剂、诱导剂、AML化疗药物处理治疗前骨髓液样本,检测并对比三基因表达情况。mRNA表达采用实时荧光定量PCR技术检测。结果 Beclin1、UCH-L5、LC3基因表达同危险度分层具有关联,且随危险度分级升高而升高(P <0. 05),初发组与和健康组比较,3种基因表达提高,缓解组与初发组比较,3种基因表达降低,差异具有统计学意义(P <0. 05)。自噬诱导剂、AML药物处理后,beclin1表达增强,UCH-L5基因表达减弱,LC-3表达增强。自噬抑制剂处理后beclin1表达减弱,UCH-L5基因表达增强,LC-3表达减弱(P<0. 05),治疗AML药物处理后beclin1表达上调UCH-L5下调,LC3上调(P <0. 05)。结论 Beclin1、UCH-L5、LC3在AML患者中呈现高表达,且与危险程度分级正相关,其表达同自噬过程关系密切,推测可通过药物调节三基因表达实现调节自噬而定目的,进而影响AML的治疗。
Objective To study the expression and function of Beclin1,UCH-L5 and LC3 in acute myeloid leukemia patients. Methods 80 patients with AML were selected and divided into 40 cases of primary group,bone marrow fluid was extracted,40 cases of remission group,after complete remission of chemical induction and drug treatment,bone marrow fluid was extracted. 40 cases of healthy group were selected and 3 genes were compared in different risk levels,health group,primary group and complete remission. The difference of mRNA expression between autophagy related genes in different risk levels was compared.Autophagy inhibitor,inducer and AML chemotherapy were used to treat bone marrow samples before treatment. The expression of three gene was detected and compared. MRNA expression was detected by real-time quantitative PCR. Results The gene expression of Beclin1,UCH-L5 and LC3 was associated with risk stratification,and increased with the risk grade( P < 0. 05). Compared with the healthy group,the expression of the 3 genes was increased,and the 3 genes were lower in the remission group than that of the primary group,and the difference was statistically significant( P < 0. 05). After autophagy inducer and AML treatment,Beclin1 expression was enhanced,UCh-L5 gene expression decreased and LC-3 expression increased. After the treatment of autophagic inhibitors,the expression of Beclin1 was weakened,the expression of UCh-L5 gene was enhanced and the expression of LC-3 was weakened( P < 0. 05). After treatment of AML,the expression of Beclin1 was down regulated and LC3 up regulated( P <0. 05). Conclusion Beclin1,UCH-L5 and LC3 are highly expressed in AML patients are positively related to the risk degree classification. The expression is closely related to the autophagy process. It is speculated that the regulation of 3 gene expression can regulate autophagy by regulating the expression of autophagy,and then affects the treatment of AML.
作者
苗玉迪
李艳春
李庆瑞
MIAO Yudi;LI Yanchun;LI Qingrui(Shaanxi Peoples Hospital,Xi'an,710068)
出处
《实用癌症杂志》
2019年第10期1574-1578,共5页
The Practical Journal of Cancer
基金
中央级公益性科研院所基本科研业务专项资金项目(编号:2016CS-69)
