摘要
目的观察血管性痴呆(VD)大鼠海马齿状回(DG)神经前体细胞(NPCs)增殖的动态变化及养血清脑颗粒的影响。方法72只Sprague-Dawley大鼠随机分为假手术组(n=24)、VD模型组(模型组,n=24)和养血清脑颗粒治疗组(治疗组,n=24),采用改良的Pulsinelli四血管阻断法制作VD大鼠模型。分别在造模术后1、2、4和8周采用Western blotting法检测Nestin的表达水平,用免疫荧光法观察海马齿状回区5-溴脱氧尿嘧啶(Brd U)和Brd U/Nestin的表达。结果模型组和治疗组各时间点大鼠Nestin、Brd U和Brd U/Nestin表达持续增多,4周时达高峰。与假手术组比较,模型组各时间点Nestin、Brd U和Brd U/Nestin表达明显增多(P<0.01);与模型组比较,治疗组各时间点Nestin、Brd U和Brd U/Nestin表达明显增多(P<0.01)。结论养血清脑颗粒可促进VD大鼠海马齿状回区NPCs增殖。
Objective To observe dynamic variation of neural precursor cells in dentate gyrus of hippocampus and effect of Yangxue Qingnao Granule on it in vascular dementia rats. Methods 72 Sprague-Dawley rats were randomly divided into sham group（n=24）, vascular dementia group（model group, n=24） and Yangxue Qingnao Granule group（treatment group, n=24）. The vascular dementia model was established with modified Pulsineli＇s four-vessel occlusion. The expression of Nestin was detected with Western blotting, the expression of 5-bromodeoxyuridine（BrdU） and BrdU/Nestin were detected with immunofluorescence in dentate gyrus of hippocampus 1, 2, 4 and 8 weeks after modeling. Results The expression of Nestin, BrdU and BrdU/Nestin increased in the model and treatment groups with time, peaked at 4weeks after modeling, and it was more than that of the sham group on all the time points（P〈0.01）. However, it was more in the treatment group than in the model group on all the time points（P〈0.01）. Conclusion Yangxue Qingnao Granule promotes the proliferation of neural precursor cells in dentate gyrus of hippocampus in vascular dementia rats.
出处
《中国康复理论与实践》
CSCD
北大核心
2015年第9期1025-1030,共6页
Chinese Journal of Rehabilitation Theory and Practice
基金
河北省高等学校科学技术研究重点项目(No.ZH2012046)
河北省级重大医学科研课题(No.zd2013087)
关键词
血管性痴呆
神经前体细胞
增殖
齿状回
海马
养血清脑颗粒
vascular dementia
neural precursor cells
proliferation
dentate gyrus
hippocampus
Yangxue Qingnao Granule
