摘要
基于表面增强拉曼光谱(SERS)的指纹图谱的高能量分辨率,以位于~630 cm^(-1)亚硫酸根的特征峰为定性和定量依据,本文开展了食品中滥用和非法添加二氧化硫的直接检测研究:一种检测流程实现了不同食品基质中二氧化硫的高灵敏检测,最低检出浓度达1 mgkg^(-1)的水平.针对实际样本的传统蒸馏法前处理流程的一些不足以及SERS检测的指纹图谱优势,本文进行了三方面显著改进:(1)碳酸钙和沸石取代氮气以提高二氧化硫的挥发提取效率;(2)草酸取代盐酸酸化蒸馏溶液,以有效避免挥发性强酸对操作环境的危害;(3)氢氧化钠溶液代替乙酸铅溶液作为吸收液,以防止二次污染.环境友好、灵敏度高和不受基质干扰等特点使得本方法有望取代经典蒸馏法和比色法,用于食品中非法添加二氧化硫类添加剂的快速高效筛查.
The direct detection of sulphur dioxide has been realized in various food matrixes with high sensitivity and high selectivity, on the basis of the integration of the high energy resolution of the finger-print spectrum of surface-enhanced Raman spectroscopy and the universal but easy-on-going pretreatment procedure. The characteristic peak of sulphur dioxide at -630 cm^-1 was applied as the qualitative and quantitative standard, which displayed a lowest detectable concentration at the 1 mg kg-1 level for the spiked food samples. The key point of the high sensitivity and selectivity is the effective pretreatment born out of the standard distillation one, which has been improved in the three parts. （1） Using CaCO3 and Zeolite instead of N2 gas as the bubbling reagent. （2） Using oxalic acid as the acidic distillation solution to eliminate the hazards from acid volatilization, such as hydrochloric acid. （3） Using diluted sodium hydroxide solution as the absorption reagent instead of lead acetate solution to avoid the secondary pollution. With the three distinguished advantages of environment friendly, high sensitivity and free of matrix interference, the proposed method has great potential to replace the traditional ones for the fast screening of the illegal or abused sulphur dioxide in food.
出处
《中国科学：化学》
CAS
CSCD
北大核心
2017年第6期794-800,共7页
SCIENTIA SINICA Chimica
基金
国家自然科学基金(编号:21473140)
福建省高校产学合作项目(编号:2016Y4012)
中央高校基本科研业务费专项资金(编号:2072016011)资助项目
关键词
表面增强拉曼光谱
快速筛查
环境友好
高灵敏
surface-enhanced Raman spectroscopy, quick-screening, environment friendly, high sensitivity
