摘要
为探讨天蚕素和溶菌酶对中华鳖Trionyx sinensis腐皮病的防治效果。本实验采用肌肉注射天蚕素(10 mg·kg^(-1))、溶菌酶(10 mg·kg^(-1))及天蚕素(6 mg·kg^(-1))和溶菌酶(5 mg·kg^(-1))混合物,观察其对鳗气单胞菌Aeromonas encheleia引起的中华鳖腐皮病的预防效果。以死亡率和相对保护率评价不同药物的保护效果;通过检测血清凝集效价、细胞吞噬功能和血清杀菌活力,比较不同药物对中华鳖免疫力和抗病能力的影响;比较庆大霉素和溶菌酶血药残留浓度,评估药物毒副作用。结果表明:天蚕素和溶菌酶二联法对中华鳖保护效果最好、免疫效果最佳,与其他组相比差异均有高度统计学意义(P<0.01);血药残留分析表明,溶菌酶在用药后第6天全部代谢完毕,庆大霉素停药第12天仍有残留。天蚕素和溶菌酶二联法预防中华鳖腐皮病效果显著优于抗生素,无药物残留和耐药副作用,并提高免疫力,避免了应用抗生素所带来的危害,值得推广应用。
In order to study the preventive effect of peptides and biological antibiotics on the skin fester disease of Trionyx sinensis caused by Aeromonas encheleia,3 different groups of cecropin( 10 mg · kg^(-1)),lysozyme( 10 mg·kg^(-1)) and mixed drugs( 6 mg·kg^(-1)cecropin and 5 mg·kg^(-1)lysozyme,respectively) were used for intramuscular injection. The mortality rate and relative percent survival were then calculated to evaluate the protective effects of different drugs. Agglutination titer of serum,phagocytosis and bactericidal activity of serum were measured to compare the effects of different drugs on immunity and disease resistance of T. sinensis. The residual concentrations of gentamicin and lysozyme were compared to evaluate drug toxic and side effects. The results showed that mixed drugs could extremely significant( P < 0. 01) protect T. sinensis and increase their immunity. Analysis of drug residue showed that lysozyme was completely metabolized after6 days,and in contrast,gentamicin still had residue until day 12 after withdrawal. Therefore,the preventive effect of mixed drugs is significantly better than antibiotics in skin fester disease of T. sinensis. In addition,mixed drugs of lysozyme and cecropin are worthy of spreading to application in virtue of no drug residue and other side effects,especially avoiding the damage caused by abuse of antibiotics.
出处
《四川动物》
北大核心
2017年第3期311-316,共6页
Sichuan Journal of Zoology
基金
北京市农业科技试验示范项目(20110821)
关键词
中华鳘
天蚕素
溶菌酶
腐皮病
预防
Trionyx sinensis
cecropin
lysozyme
skin fester disease
prevention
