摘要
为研究蒙花苷与木犀草素联用对SD大鼠离体血管环的舒张作用及其作用机制。采用离体血管环张力实验观察蒙花苷单用(7.5×10^(-6)g·mL^(-1))、木犀草素单用(7.5×10^(-6)g·mL^(-1))、蒙花苷与木犀草素联用(1∶4)对去甲肾上腺素引起的SD大鼠完整、去内皮、L-NAME及吲哚美辛预处理胸主动脉收缩的影响;Western blot检测胸主动脉p-Akt,p-eNOS等蛋白水平。实验结果显示,蒙花苷与木犀草素联用对完整、去内皮及L-NAME预处理的血管环舒张率高于两者单用;且两者联用能显著促进血管中p-Akt,p-eNOS蛋白表达。实验结果说明蒙花苷与木犀草素联用能有效舒张血管,其机制可能是通过激活PI3K/Akt/NO信号通路,增强eNOS活性,从而增加NO的合成与释放,达到舒张血管的作用。
To study the relaxation effect of buddleoside combined with luteolin on aortic rings in SD rats and its mechanism. The effect of buddleoside alone（7.5×10^-6g·mL^-1）, luteolin alone（7.5×10^-6g·mL^-1） and the combination of buddleoside and luteolin（1：4） on norepinephrine-induced contractility of complete, endothelium-denuded, and L-NAME and indomethacin-pretreated thoracic aorta in SD rats were observed in the in vitro ring tension test. Western blot was used to detect p-Akt and p-eNOS protein expressions in the thoracic aorta. The experimental results showed that buddleoside combined with luteolin could significantly increase the relaxation rate of blood vessels and endothelium and L-NAME-pretreated vascular rings compared with the two single administrations. And buddleoside combined with luteolin could also significantly increase p-Akt and p-eNOS protein expressions.The results suggested that the combination of buddleoside and luteolin could effectively relax the blood vessel, and the mechanism may be to increase the synthesis and release of NO and reach the role of relaxing blood vessel by activating PI3K/Akt/NO signaling pathway and enhancing the activity of eNOS.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2017年第7期1370-1375,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81274123)
关键词
蒙花苷
木犀草素
联用
血管舒张
机制
高血压
buddleoside
luteolin
combination
vasorelaxation
mechanism
hypertension
