摘要
[目的]研究独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇的最佳提取条件,同时建立了同时测定独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇含量的高效液相色谱法(HPLC)。[方法]通过单因素分析法考察了提取溶剂、料液比、超声提取时间对独活提取物中二氢欧山芹醇-β-D-葡萄糖苷和二氢欧山芹醇含量的影响。采用HPLC法对不同提取条件和不同产地的独活中两种成分含量。[结果]独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇的最佳提取条件为提取溶剂用100%的甲醇,料液比为1∶50,超声提取60min。二氢欧山芹醇-β-D-葡萄糖苷在0.3125~20μg/m L内呈良好线性,平均回收率为99.0%(RSD=2.99%);二氢欧山芹醇在0.3125~20μg/m L内呈良好线性,平均回收率为102%(RSD=2.59%)。[结论]所建立的高效液相色谱法,能够准确测定独活中二氢欧山芹醇-β-D葡萄糖苷和二氢欧山芹醇含量,可用于独活药材的质量评价。
[Objective] To research the best extraction process about columbianetin-β-D-glucopyranoside and columbianetin inAngelicae pubescentis radix and develop a simple, accurate, repeatable method for simultaneous determination of columbianetin-β-D- glucopyranoside and columbianetin in Angelicae pubescentis radix by High Performance Liquid Chromatography （HPLC）. [Methods] The sample was extracted by different extraction solvent, extraction time, ratio of liquid to material to analysis the effect about the content of columbianetin-β-D-glucopyranoside and columbianetin. [Results] The optimum level were methanol, 60 min, 1-50, respectively. Two calibration curves exhibited good linearity （r=1） within the linear range from 0.312 5 μg/mL to 20 μg/mL. The average percentages of recovery was 99.0% and 102%, and the RSD was 2.99% and 2.59%, respectively. [Conclusion] The developed simple HPLC method could successfully be utilized to determine two components in samples from different regions. It was concluded that this new method could determine columbianetin-β-D-glucopyranoside and columbianetin for quality control of Angelicae pubescentis radix.
作者
张璐
葛园园
李晋
常艳旭
ZHANG Lu GE Yuan-yuan LI Jin CHANG Yan-xu(Tianjin State Key Laboratory ofModern Chinese Medicine, Tianjin University ofTraditional Chinese Medicine, Tianjin 300193, China)
出处
《天津中医药》
CAS
2017年第5期345-348,共4页
Tianjin Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年项目(81503213)
