摘要
目的:建立独活中总香豆素含量的测定方法。方法:以蛇床子素为对照品,采用紫外分光光度法测定独活中总香豆素含量。结果:检测波长为322nm,蛇床子素质量浓度在2～12mg/mL范围内与吸光度呈良好线性关系(r=0.9996),平均回收率99.18%(RSD=1.69%,n=6)。结论:本方法简便易行,结果稳定可靠,可用于独活及其制剂的质量控制。
Objective：To establish the method for content determination of total coumarins in Radix Angelicae Pubescentis.Methods：The content of the total coumarins was determined by UV spectrophotometry.Results： The optimal detective wavelength was 322nm for the total coumarins.Satisfactroy linear relationship with the absorbability was achieved when the mass concentration of osthole was within a range of 2～12mg/mL（r= 0.9996）.The average recovery rate was 99.18%（RSD = 1.69%,n= 6）.Conclusion：Using UV spectrophotometry to detect the total coumarin content is simple,accurae and sensitive,and it can be used for the quality control of Radix Angelicae Pubescentis.
出处
《中华中医药学刊》
CAS
2010年第12期2647-2648,共2页
Chinese Archives of Traditional Chinese Medicine
关键词
独活
香豆素类
含量测定
分光光度法
Radix Angelicae Pubescentis
Coumarins
Content determination
Spectrophotometry
