摘要
采用发酵法制备肉独活化妆品原料液,以蛇床子素含量为菌株的筛选指标,分别对发酵温度、发酵时间、碳源添加量、接种量进行单因素试验,进而用正交试验优化制备工艺,并用脂氧合酶(5-LOX)抑制率评价其抗炎活性。结果表明,鼠李糖乳杆菌为制备肉独活发酵液的适宜菌株,其优化工艺为:发酵温度37℃、发酵时间40 h、碳氮比为6∶1,葡萄糖质量浓度为8 mg/mL、接种量为体积分数0.5%,该条件下制备的肉独活原料液的蛇床子素质量浓度为79.50 mg/L,5-LOX抑制率为84.12%。
The fermentation technology for preparing Angelica pubescens broth applied in cosmetics was studied in this study.The content of osthole in fermentation broth was used as the main index to screening fermentation strain.The singlefactor experiments were carried out on fermentation temperature,fermentation time,addition amount of carbon source and inoculation amount,then the orthogonal experiment was used to optimize the fermentation process,and the inhibition rate of lipoxygenase(5-LOX)was used as the index to evaluate its anti-inflammatory activity.Results showed that Lactobacillus rhamnosus was the optimized strain for preparing Angelica pubescens fermentation broth,the optimal fermentation technology was as follows:fermentation temperature 37℃,fermentation time 40 h,mass ratio of carbon and nitrogen 6∶1,the total amount of glucose 8 mg/mL and the inoculation amount 0.5%(volume fraction).With these conditions,the concentration of osthole in the fermentation broth was 79.50 mg/L,its lipoxygenase inhibition ratio was 84.12%.
作者
黄煜玲
袁亚涛
吉倪暄
李雪梅
孟德旭
管世敏
荣绍丰
HUANG Yuling;YUAN Yatao;JI Nixuan;LI Xuemei;MENG Dexu;GUAN Shimin;RONG Shaofeng(School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China)
出处
《香料香精化妆品》
CAS
2022年第2期75-81,共7页
Flavour Fragrance Cosmetics
关键词
肉独活
蛇床子素
鼠李糖乳杆菌
发酵
Angelica pubescens
osthole
Lactobacillus rhamnosus
fermentation
