摘要
目的:腰痛丸(Ⅰ)是盐城市中医院的院内中药复方制剂,具有良好的临床治疗效果。利用高效液相色谱法(HPLC),建立同时测定腰痛丸(Ⅰ)中蛇床子素、升麻素苷及橙皮苷含量的方法。方法:采用Waters CAPCELL PAK C18色谱柱(4.6 mm×250 mm,5μm);以流动相为0.2 mol·L^(-1)醋酸铵溶液(A)-甲醇(B),梯度洗脱;柱温30℃;检测波长284 nm(升麻素苷、橙皮苷)、324 nm(蛇床子素);流速为1.0 mL·min^(-1);进样量为20μL。结果:经方法学验证,在50 min内,腰痛丸(Ⅰ)中蛇床子素、升麻素苷和橙皮苷峰分离度良好;峰面积与其浓度呈良好的线性关系,回收率符合规定,稳定性良好,检测方法可靠。结论:该含量测定方法简便快速、结果精确可靠并且重现性好,可作为腰痛丸(Ⅰ)中蛇床子素、升麻素苷和橙皮苷的质量控制方法。
Objective:To establish an high performance liquid chromatography(HPLC)method to determine osthole,prim-O-glucosylcimifugin and hesperidin in a hospital preparation Low Back Pain Pills(Ⅰ).Methods:A Waters CAPCELL PAK C18 column(4.6 mm×250 mm,5μm)was adopted to separate the three contents by ammonium acetate(0.2 mol·L^(-1))-methanol as mobile phase with UV detection at 284 nm for prim-o-glucosylcimifugin and hesperidin,324 nm for osthole.The flow rate was 1.0 mL·min^(-1 )and the column temperature was 30℃.Results:Osthole,prim-O-glucosylcimifugin and hesperidin in the Low Back Pain Pills(Ⅰ)were completely separated within 50 min.Regression equations showed good linear relationships between peak-area and content of each compound obtained.Conclusion:Due to its convenience,accuracy,high sensitivity and good repetition,this can be used for quality control of Low Back Pain Pills(Ⅰ).
作者
仇其原
张卓
朱干红
QIU Qiyuan;ZHANG Zhuo;ZHU Ganhong(Yancheng Food and Drug Supervision and Inspection Center,Yancheng 224000,China;Yancheng Traditional Chinese Medicine Hospital,Yancheng 224000,China)
出处
《药学与临床研究》
2022年第2期116-118,共3页
Pharmaceutical and Clinical Research
