摘要
目的:探讨胃癌术前CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达的关系。方法:收集榆林市第二医院收治的胃癌患者68例作为观察组,根据肿瘤分期分为早中期胃癌组41例、晚期胃癌组27例;取同期在本院就诊被证实为胃良性病变的患者50例作为胃良性病变组。采用CT扫描检测三组患者的CT强化率、灌注参数,采用酶联免疫吸附法(ELISA)检测血清肿瘤标志物含量,采用RT-PCR法检测病灶组织增殖基因mRNA表达量。结果:晚期胃癌组的CER、AF、BV、CL水平高于早中期胃癌组、胃良性病变组(P<0.05);晚期胃癌组的血清CA72-4、CA19-9、CA125、CEA含量高于早中期胃癌组、胃良性病变组(P<0.05);晚期胃癌组的组织CADM1、miRNA-34a、Cystatin M mRNA表达量低于早中期胃癌组、胃良性病变组,Survivin、I2PP2A mRNA表达量高于早中期胃癌组、胃良性病变组(P<0.05)。经Pearson检验发现,胃癌患者的CT强化率、灌注参数与血清肿瘤标志物含量、肿瘤病灶增殖分子表达量存在直接相关关系。结论:胃癌术前CT强化率、灌注参数与肿瘤恶性程度直接相关,可作为远期肿瘤诊断及恶性程度判断的可靠手段。
Objective：To study the relationship of preoperative gastric cancer CT enhancement ratio and perfusion parameters with serum tumor marker levels and proliferation molecule expression in tumor lesions.Methods：A total of 68 patients with gastric cancer treated in the Second Hospital of Yulin City between May 2012 and May 2016 were chosen as observation group and sub-divided into early and middle gastric cancer group（n=41）and advanced gastric cancer group（n=27）according to the tumor stage;50 patients diagnosed with benign gastric diseases in our hospital during the same period were selected as benign gastric lesion group.CT enhancement rate and perfusion parameters of three groups of patients were detected by CT scan,serum tumor marker levels were evacuated by enzyme-linked immunosorbent assay（ELISA）,and the proliferation gene mRNA expression levels were detected by RT-PCR method.Results：CER,AF,BV and CL levels of advanced gastric cancer group were higher than those of early and middle gastric cancer group and benign gastric lesion group（P〈0.05）;serum CA72-4,CA19-9,CA125 and CEA contents of advanced gastric cancer group were higher than those of early and middle gastric cancer group and benign gastric lesion group（P〈0.05）;CADM1,miRNA-34 aand Cystatin M mRNA expression in tissue of advanced gastric cancer group were lower than those of early and middle gastric cancer group and benign gastric lesion group while Survivin and I2PP2A mRNA expression were higher than those of early and middle gastric cancer group and benign gastric lesion group（P〈0.05）.The Pearson test showed that the CT enhancement rate and perfusion parameters in patients with gastric cancer are directly correlated with the serum tumor marker levels and the proliferation gene expression in tumor lesions.Conclusion：Preoperative gastric cancer CT enhancement rate and perfusion parameters are directly related to the tumor malignancy,and can be used as a reliable method for the long-term tumor diagnosis and malignancy ju
出处
《海南医学院学报》
CAS
2017年第6期823-826,共4页
Journal of Hainan Medical University
基金
基金项目:陕西省教育厅(2010H25)~~
关键词
胃癌
计算机断层扫描
肿瘤标志物
增殖分子
Gastric cancer
Computed tomography
Tumor marker
Proliferation molecule
