摘要
目的:针对小球藻细胞壁异常坚韧的特点,探讨小球藻蛋白的制备工艺及蛋白水解肽的抗氧化效果。方法:小球藻溶胀与稀碱结合工艺,借助minitab统计软件,利用中心组合试验法对小球藻蛋白的提取工艺条件进行优化;采用复合风味蛋白酶对所制备的小球藻蛋白进行酶解,用体外抗氧化方法筛选出抗氧化效果最强的酶解组分,体内采用秀丽隐杆线虫H_2O_2抗性试验探讨水解肽的抗氧化能力。结果:小球藻经溶胀过夜处理后,采用3.9%氢氧化钠溶液作溶剂,在料液比1:30、提取温度47℃条件下提取27 min,小球藻蛋白的得率为5.13%。小球藻蛋白按复合风味蛋白酶与底物的质量比([E]/[S])=1∶25,在50℃、pH 6.0条件下酶解4 h,所得水解肽对DPPH·清除率达68.93%,对羟自由基清除率达82.00%,还原力为20.42,能显著增强线虫寿命及其对H_2O_2的抗性(P<0.05)。结论:碱法提取制备的小球藻蛋白,经酶解获得的水解肽具有较好的抗氧化能力。
Objective：To investigate the process of Chlorella protein effectively dissolving out according to the characteristics of its cell wall abnormalities leathery,and the antioxidant effect of the hydrolyzed peptides.Methods：This experiment used the process with Chlorella swelling and dilute alkali method,and optimized the technological conditions of extraction which used NaOH to extracting the Chlorella protein by the central combination testing method with the aid of minitab the statistical software.The Chlorella protein was hydrolyzed by flavourzyme at the optimum enzymolysis conditions,and the strongest antioxidant effect of enzymatic hydrolysis was selected using in vitro antioxidant methods.The antioxidant effect of the hydrolysis peptide was investigated further by the H2O2 resistance experiment of Caenorhabditis elegans in vivo.Results：Chlorella was broken pretreatment by swelling overnight,then using 3.9% NaOH solution as solvent,and extraction of 27 min at 47 ℃,with 1∶30 liquid ratio.The extraction yield was 5.13% under this extraction conditions.The Chlorella protein was hydrolyzed for 4 h at 50 ℃,with the ratio of flavourzyme and substrate ratio （[E]/[S]）=1∶25 in pH 6.0,and the hydrolysis of peptides on DPPH· scavenging rate reached 68.93%,on hydroxyl radical scavenging rate reached 82%,and the reducing power reached 20.42.The hydrolysis of peptides can significantly enhance the Caenorhabditis elegans life and resistance to H2O2 （P〈0.05）.Conclusion：The hydrolysis of peptides obtained from Chlorella protein had an excellent antioxidation ability.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第2期92-100,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
"十二五"农村领域国家科技计划(No.2013BAD10B02)
关键词
小球藻蛋白
响应面优化
水解肽
抗氧化
Chlorella protein
response surface optimization
hydrolysis of peptides
antioxidant
