摘要
目的:针对小球藻丰富的蛋白质含量,酶解制备小球藻蛋白水解肽,与铁(Ⅲ)合成小球藻水解肽铁配合物(CPIC),并考察CPIC的理化性质。方法:碱法结合超声的提取工艺制备小球藻蛋白质,进而采用碱性蛋白酶对所制备的小球藻蛋白进行酶解,酶解产物与三氯化铁作用合成CPIC,并测定CPIC的理化性质。结果:碱法结合超声的提取工艺获得的小球藻蛋白质的得率为11.28%,小球藻蛋白按碱性蛋白酶与底物浓度比为5%,在p H=9.0,温度为55℃的条件下水解2h,水解度达34.89%。酶解产物与三氯化铁作用合成CPIC为无定形棕色粉末,易溶于水,其水溶液中不存在游离的铁(Ⅲ),表明铁(Ⅲ)与水解肽形成了稳定的配合物。采用抗坏血酸还原,CPIC中的铁(Ⅲ)易被抗坏血酸还原成铁(Ⅱ)。结论:小球藻水解肽铁配合物有望开发成理想的口服补铁剂。
Objective： To investigate the process of Chlorella protein effectively dissolving out, and enzymatic preparation of Chlorella protein hydrolysis peptides according to the rich protein content of Chlorella. Then to obtaine the Chlorella protein hydrolysis peptides-Fe complex （CPIC） and analyse its physicochemical property. Methods： The Chlorella proteins were prepared by the process with ultrasonic and dilute alkali extraction, and the proteins were hydrolyzed by alkali protease at the optimum enzymolysis conditions. Then Chlorella protein hydrolysis peptides-Fe complex （CPIC） were synthesized with the hydrolysis peptides and FeCI3, and its physicochemical property was determined. Results： The extraction yield of the Chlorella protein was 11.28% with ultrasonic and dilute alkali extraction. The Chlorella protein was hydrolyzed for 2 h at 55 %, with the ratio of flavourzyme and substrate ratio （Ⅲ/[S]）=5% in pH9.0, and the hydrolysis degree was 34.89%. CPIC was a kind of brownish and shapeless powder, and was able to dissolve in water. Free Fe（Ⅲ） did not exist in the aqueous solution. CPIC was very stable and could be reduced to Fe（Ⅱ） by ascorbic acid. Conclusion： CPIC would be expected to become an ideal iron-supplementary nutrition.
出处
《食品科技》
CAS
北大核心
2015年第4期334-338,共5页
Food Science and Technology
基金
"十二五"农村领域国家科技计划项目(2013BAD10B02)
浙江省大学生科研创新团队项目(2014R409032)
关键词
小球藻蛋白
水解肽
配合物
补铁剂
Chlorella protein
hydrolysis of peptides
complex
iron-supplementary
