摘要
急性过量饮酒会导致急性酒精中毒,引起胃出血、诱发心脑血管等疾病,已成为临床上最为常见的症状之一。目前治疗急性酒精中毒的合成药物会对身体产生副作用,急需发掘一种天然活性物质可代替人工合成药物。选择钝顶螺旋藻多糖作为研究对象,探讨螺旋藻多糖对急性酒精中毒损伤的影响。将30只小鼠作为处理对象随机分为空白、模型、阳性对照、低剂量及高剂量组,每组连续灌胃给药21 d(1次/d);第22天除正常组外,其余组通过灌胃方式给予12 mL/kg的50%乙醇,12 h后处死,解剖肾、心、肝及胸腺,研磨取上清,通过检测各组织中过氧化氢酶、谷胱甘肽、丙二醛以及肝中丙氨酸氨基和天冬氨酸氨基转移酶活性,探究钝顶螺旋藻多糖对急性酒精中毒后生理指标的影响。结果表明,与模型组相比,螺旋藻多糖组中的过氧化氢酶和谷胱甘肽含量有显著提升,丙二醛含量显著降低;肝组织中,丙氨酸氨基和天冬氨酸氨基转移酶含量明显下降,恢复至阳性对照组水平。研究结果为治疗急性酒精性中毒引起的肾、心、肝及胸腺的氧化损伤提供了科学依据。
Consuming large quantities of alcohol over a short time can lead to acute alcoholism, which usually causes gastrorrhagia and cardiovascular disease. These diseases have become the most common clinical symptoms. Recently, synthetic drugs used to treat acute alcoholism have side effects on the body. Therefore, it is urgent to discover some natural active substances that can replace synthetic drugs. The goal of this article is to study the protective effect of polysaccharide from spirulina on acute alcoholism. Thirty mice were randomly divided into control(blank), model(alcohol injury), positive control, treatments(low PSP and high PSP dose). The low PSP and high PSP dose treatments were continuously taking polysaccharide from spirulina for 21 days(1 time/d). The positive control was given a continuous bifendate pills for 21 days(1 time/d). The blank control and alcohol injury were given solvent(water) for 21 days(1 time/d). After 22 days, except for the control group, others were given 50% alcohol(12 mL/kg) by means of intragastric. All groups mice were killed 12 hours later, and then the hearts, kidneys, thymus and livers were dissected from mice. The tissues were ground and then the supernatants were collected. The activities of catalase(CAT), glutathione(GSH), malondialdehyde(MDA), alanine aminotransferase(ALT) and aspartic aminotransferase(AST) were analyzed. The results showed that compared with the model group, the contents of CAT and GSH in treatments increased significantly and the content of MDA decreased dramatically. In liver tissue, the contents of ALT and AST decreased distinctly and returned to the level of positive control group. The above results would provide a scientific basis for the treatment of oxidative damage in the kidney, heart, liver and thymus caused by acute alcoholism.
作者
陈帅行
马浩天
李润植
葛保胜
崔红利
CHEN Shuai-hang;MA Hao-tian;LI Run-zhi;GE Bao-sheng;CUI Hong-li(Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University,Taigu 030801;Bioengineering Center,China University of Petroleum(Huadong),Qingdao 266580,China)
出处
《生物学杂志》
CAS
CSCD
北大核心
2020年第3期54-57,共4页
Journal of Biology
基金
国家重点研发计划项目(2016YFE0106700)
中国博士后科学基金(2018M631768)
山西省应用基础研究(青年科技研究基金)项目(201801D22125)
山西省重点研发计划一般项目(社会发展方面)(201803D31063)
山西省煤基重大科技专项(FT-2014-01)
山西省重点研发重点项目(201603D312005)。
关键词
钝顶螺旋藻多糖
急性酒精中毒
器官
氧化损伤
保护作用
polysaccharide Spirulina platensis(PSP)
acute alcoholism
organs
oxidative injury
protective effects
