摘要
目的 探讨膜联蛋白A1（ANXA1）与增殖细胞核抗原（PCNA）在胆管癌组织中的表达并分析其临床意义.方法 采用免疫组织化学技术检测ANXA1与PCNA在70胆管癌组织和15例非肿瘤胆道上皮组织中的表达.结果 免疫组织化学结果显示70例胆管癌、15例非肿瘤胆道上皮组织中ANXA1与PCNA阳性表达率分别为54.3%、13.3%和77.1%、33.3%.ANXA1与PCNA蛋白在胆管癌中的表达均明显高于非肿瘤胆道上皮组织（x2=11.170,P=0.001;x2=8.320,P=0.004）.ANXA1蛋白表达与胆管癌的临床分期和组织学分级明显相关（x2=5.090,P=0.024;x2=6.090,P=0.047）;胆管癌组织中PCNA蛋白表达与临床分期和组织学分级明显相关（x2=13.010,P=0.001;x2=6.660,P=0.017）.ANXA1与PCNA表达呈正相关（r=0.250,P=0.036）.结论 ANXA1与PCNA是反映胆管癌发生发展的重要生物学指标.
Objective To investigate the expression of annexin A1 （ANXA1） and proliferating cell nuclear antigen （PCNA） in cholangiocarcinoma tissues and its clinical significance.Methods Immunohistochemical staining was used to detect the expression of ANXA1 and PCNA in 70 cases of cholangiocarcinoma and 15 cases of non-tumor bile duct epithelium.Results The expression of ANXA1 and PCNA in cholangiocarcinoma （54.3% and 77.1%） was significantly higher than that in non-tumor bile duct epithelium （13.3% and 33.3%;x2=8.320, P=0.004;x2=11.170,P=0.001）.ANXA1 expression was significantly correlated with clinical stage and tumor grade.The positive rate of ANXA1 was significantly higher in the stage Ⅲ＋Ⅳ group and moderately or poorly-differentiated group than that in stage Ⅰ＋Ⅱ group and well-differentiated group （x2=5.090, P=0.024;x2=6.090, P=0.047）.The PCNA expression was significantly correlated with clinical stage and tumor grade.The positive rate of PCNA was significantly higher in the stage Ⅲ＋Ⅳ group and moderately or poorly-differentiated group than that in stage Ⅰ＋Ⅱ group and well-differentiated group （x2=13.010, P=0.001;x2=6.660, P=0.017）.A significant correlation was found between the expression of ANXA1 and PCNA in cholangiocarcinoma （r=0.250, P=0.036）.Conclusion ANXA1 and PCNA may be important biomarkers in carcinogenesis and progression of cholangiocarcinoma.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2017年第3期467-469,共3页
Chinese Journal of Experimental Surgery
关键词
胆管癌
膜联蛋白A1
增殖细胞核抗原
Cholangiocarcinoma
Annexin A1
Proliferation cell nuclear antigen
