摘要
目的研究膜联蛋白A1(annexim A1,Anx A1)和表皮生长因子受体(EGFR)在食管鳞癌组织中的表达及其相关性。方法应用免疫组化SABC法检测48例食管鳞癌,35例癌前病变(21例低级别上皮内瘤变,14例高级别上皮内瘤变)和18例正常对照组织中Anx A1和EGFR的表达,并结合其临床病理资料进行分析。结果正常食管鳞状上皮组织中Anx A1的阳性表达率(100%)明显高于低级别上皮内瘤变组织(57.1%)、高级别上皮内瘤变组织(7.1%)和食管鳞癌组织(6.3%),差异均有统计学意义(P<0.05)。正常食管鳞状上皮组织中EGFR的阳性表达率(0.0%)明显低于低级别上皮内瘤变组织(47.6%)、高级别上皮内瘤变组织(64.3%)和食管鳞癌组织(72.9%),差异也均有统计学意义(P<0.05)。食管鳞癌组织中Anx A1的表达降低,EGFR的表达升高,两者之间的表达强度呈负相关(P<0.001)。结论 Anx A1的低表达和EGFR的高表达可能为食管鳞癌的早期诊断提供依据。
Objective To investigate the expression of Anx AI and EGFR in human esophageal squamous cell carcinoma （ESCC） tissue and their relationship. Methods Immunohistochemical staining was used to detect the expression of Anx A1 and EGFR in normal esophageal squamous epithelium （ n = 18 ）, low-grade intraepithelial neoplasia （ n = 21 ）, high- grade intraepithelial neoplasia （ n = 14） and ESCC （ n = 48）. The clinicopathologic features of ESCC were analyzed. Results The positive rate of Anx A1 in normal squamous epithelia of esophagus （ 100.0% ） was significantly higher than that in lowgrade intraepithelial neoplasia （57.1%）, high-grade intraepitheial neoplasia （7.1%） and ESCC （6.3%） （ P 〈 0.05）. However, the positive rate of EGFR in normal squamous epithelia of esophagus （0.0%） was significantly lower than that in low-grade intraepthelial neoplasia （47.6%）, high-grade intraepithelial neoplasia （64.3%） and ESCC （72.9%） （ P 〈 0. 05）. Morever, in ESCC tissue, Anx A1 and EGFR manifested negative correlation （ r = -0. 472, P 〈0.01 ）. Conclusion The low expression of Anx A1 and the high expression of EGFR may indicate early esophageal oncogenesis.
出处
《临床消化病杂志》
2011年第3期152-155,共4页
Chinese Journal of Clinical Gastroenterology
关键词
食管鳞状细胞癌
膜联蛋白A1
表皮生长因子受体
免疫组织化学
Esophageal squamous cell carcinoma
Annexin A1
Epithelial growth factor receptor
hnmunohisto- chemistry
